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Titolo:
MOLECULAR ANALYSIS OF 2 SCRR REPRESSORS AND OF A SCRR-FRUR HYBRID REPRESSOR FOR SUCROSE AND D-FRUCTOSE SPECIFIC REGULONS FROM ENTERIC BACTERIA
Autore:
JAHREIS K; LENGELER JW;
Indirizzi:
UNIV OSNABRUCK,FACHBEREICH BIOL CHEM,ARBEITSGRP GENET,POSTFACH 4469 W-4500 OSNABRUCK GERMANY UNIV OSNABRUCK,FACHBEREICH BIOL CHEM,ARBEITSGRP GENET,POSTFACH 4469 W-4500 OSNABRUCK GERMANY
Titolo Testata:
Molecular microbiology
fascicolo: 1, volume: 9, anno: 1993,
pagine: 195 - 209
SICI:
0950-382X(1993)9:1<195:MAO2SR>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI K-12; PROTEIN-DNA RECOGNITION; LAC REPRESSOR; KLEBSIELLA-PNEUMONIAE; PHOSPHOTRANSFERASE SYSTEM; SALMONELLA-TYPHIMURIUM; NUCLEOTIDE-SEQUENCE; CLONING VEHICLES; GENE-SCRA; METABOLISM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
60
Recensione:
Indirizzi per estratti:
Citazione:
K. Jahreis e J.W. Lengeler, "MOLECULAR ANALYSIS OF 2 SCRR REPRESSORS AND OF A SCRR-FRUR HYBRID REPRESSOR FOR SUCROSE AND D-FRUCTOSE SPECIFIC REGULONS FROM ENTERIC BACTERIA", Molecular microbiology, 9(1), 1993, pp. 195-209

Abstract

The scr regulon of pUR400 and the chromosomally encoded scr regulon Of Klebsiella pneumoniae KAY2026 are both negatively controlled by a specific repressor (ScrR). As deduced from the nucleotide sequences, both scrR genes encode polypeptides of 334 residues (85.5% identical basepairs, 91.3% identical amino acids), containing an N-terminal helix-turn-helix motif. Comparison with other regulatory proteins revealed 30.6% identical amino acids to FruR, 27.0% to Lacl and 28.1% to GalR. Six scrR(s) super-repressor mutations define the inducer-binding domain. The scr operator sequences were identified by in vivo titration testsof the sucrose repressor and by in vitro electrophoretic mobility shift assays. D-fructose, an intracellular product of sucrose transport and hydrolysis, and D-fructose 1-phosphate were shown to be molecular inducers of both scr regulons. An active ScrR-FruR hybrid repressor protein was constructed with the N-terminal part of the sucrose repressorof K. pneumoniae and the C-terminal part of the fructose repressor ofSalmonella typhimurium LT2. Gel retardation assays showed that the hybrid protein bound to scr-specific operators, and that D-fructose 1-phosphate, the inducer for FruR, was the only inducer. In vivo, neither the operators of the fru operon nor of the pps operon, the natural targets for FruR, were recognized, but the scr operators were. These dataand the data obtained from the super-repressor alleles confirm previous models on the binding of repressors of the Lacl family to their operators.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 04:52:59