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Titolo:
SPECIFIC MITOCHONDRIAL PROTEINS IN POLLEN - PRESENCE OF AN ADDITIONALATP SYNTHASE BETA-SUBUNIT
Autore:
DEPAEPE R; FORCHIONI A; CHETRIT P; VEDEL F;
Indirizzi:
UNIV PARIS 11,GENET MOLEC PLANTES LAB,CNRS,UNITE RECH ASSOCIEE 1128,BATIMENT 360 F-91405 ORSAY FRANCE UNIV PARIS 11,STRUCT & METAB PLANTES LAB,CNRS,UNITE RECH ASSOCIEE 1128 F-91405 ORSAY FRANCE
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 13, volume: 90, anno: 1993,
pagine: 5934 - 5938
SICI:
0027-8424(1993)90:13<5934:SMPIP->2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOPLASMIC MALE-STERILITY; POLYACRYLAMIDE GELS; ZEA-MAYS; MAIZE; ELECTROPHORESIS; POLYPEPTIDES; GENES; BIOGENESIS; SEPARATION; SEQUENCE;
Keywords:
MALE GAMETOGENESIS; POLLEN MITOCHONDRIA ISOLATION; 2-DIMENSIONAL PROTEIN ELECTROPHORESIS; INORGANELLO PROTEIN SYNTHESIS; PROTEIN ISOFORMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
R. Depaepe et al., "SPECIFIC MITOCHONDRIAL PROTEINS IN POLLEN - PRESENCE OF AN ADDITIONALATP SYNTHASE BETA-SUBUNIT", Proceedings of the National Academy of Sciences of the United Statesof America, 90(13), 1993, pp. 5934-5938

Abstract

A protocol was designed to obtain a pure fraction of pollen mitochondria from the diploid species Nicotiana sylvestris, the female parent of the allotetraploid Nicotiana tabacum. Most organelles were morphologically intact and able to perform in organello mitochondrial (mt) protein synthesis. As revealed by two-dimensional protein electrophoresis,numerous quantitative differences exist between leaf and pollen mt proteins. Moreover, additional mt polypeptides, named R (for reproductive), encoded by either nuclear or mitochondrial genes, are found in pollen. The most abundant R polypeptide, R1 (M(r) 53,000, pI 5.6), is nuclearly encoded, is membrane bound, and cross-reacts with an antibody directed against the beta subunit of the mt ATP synthase (ATPase). N-terminal microsequence analysis showed that the two ATPase beta subunitspresent in leaves (beta1 and beta2) and the R1 pollen-specific subunit are encoded by distinct genes. A similar additional ATPase beta subunit was observed in pollen mitochondria from Petunia, suggesting that this polypeptide is of general importance for male gametophytic development in Solanaceaes.

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Documento generato il 29/11/20 alle ore 06:41:13