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Titolo:
FURTHER CHARACTERIZATION OF 5-HT-RECEPTOR AND 5-HT(3)-RECEPTOR AGONISTS-STIMULATED PHOSPHOINOSITOL PHOSPHATES ACCUMULATION
Autore:
EDWARDS E; ASHBY CR; WANG RY;
Indirizzi:
UNIV MARYLAND,DEPT PHARMACOL & TOXICOL,20 N PINE ST BALTIMORE MD 21201 SUNY,DEPT PSYCHIAT & BEHAV SCI STONY BROOK NY 11794
Titolo Testata:
Brain research
fascicolo: 1, volume: 617, anno: 1993,
pagine: 113 - 119
SICI:
0006-8993(1993)617:1<113:FCO5A5>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; RAT CEREBRAL-CORTEX; INOSITOL PHOSPHOLIPID HYDROLYSIS; ISLET-ACTIVATING PROTEIN; PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE; CALCIUM REQUIREMENT; SIGNAL TRANSDUCTION; ARACHIDONIC-ACID; CORTICAL SLICES; PERTUSSIS TOXIN;
Keywords:
POLYPHOSPHOINOSITIDE; PHOSPHOINOSITIDE HYDROLYSIS; SEROTONIN (5-HT); 2-METHYL-SEROTONIN (2-ME-5-HT); PHENYLBIGUANIDE; CALCIUM; CALCIUM IONOPHORE; PHORBOL ESTER; PROTEIN KINASE-C; PERTUSSIS TOXIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
E. Edwards et al., "FURTHER CHARACTERIZATION OF 5-HT-RECEPTOR AND 5-HT(3)-RECEPTOR AGONISTS-STIMULATED PHOSPHOINOSITOL PHOSPHATES ACCUMULATION", Brain research, 617(1), 1993, pp. 113-119

Abstract

The calcium requirement for serotonin (5-HT)- and the 5-HT3 receptor agonists, 2-Me-5-HT- and PBG-dependent breakdown of phosphatidyl inositol has been examined in the rat fronto-cingulate cortex. The omissionof added Ca2+ from the Kreb's incubation medium reduced the [H-3]inositol phosphate accumulation from pre-labelled phospholipids. Removal of Ca2+ by pre-incubation with EGTA (0.5 mM), as well as the addition of the calcium channel blocker, lanthanum (10 muM), abolished the 5-HT-and the 5-HT3 receptor agonists'-stimulated phosphoinositide (PI) response. By contrast, the calcium ionophores, A 23187 and Ionomycin (both at 30 muM) stimulated PI hydrolysis, and this effect was additive tothe increased PI turnover induced by 5-HT, 2-Me-5-HT and PBG. The increase in phosphoinositide hydrolysis induced by 5-HT and 2-Me-5-HT wassignificantly inhibited by phorbol dibutyrate (PDBu) and phorbol myristate acetate, indicating that the activation of protein kinase C (PKC) may provide negative feedback to the PI response induced by 5-HT and2-Me-5-HT. The effect of PDBu on 2-Me-5-HT-stimulated PI metabolism was reversed by the PKC inhibitors, staurosporine, calphostin C and chelerythrine (all at 10 muM), however, Pertussis toxin (0.5 and 1 mug) had no effect on either 5-HT's or 2-Me-5-HT's increased stimulation of PI hydrolysis, suggesting that this response is not associated to a G(i) GTP binding protein.

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Documento generato il 13/07/20 alle ore 19:43:18