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Titolo:
ROLE OF CATION GRADIENTS IN HYPERCONTRACTURE OF MYOCYTES DURING SIMULATED ISCHEMIA AND REPERFUSION
Autore:
NISHIDA M; BORZAK S; KRAEMER B; NAVAS JP; KELLY RA; SMITH TW; MARSH JD;
Indirizzi:
WAYNE STATE UNIV,421 E CANFIELD AVE,RM 1107 DETROIT MI 48201 BRIGHAM & WOMENS HOSP,DIV CARDIOVASC BOSTON MA 02115 BRIGHAM & WOMENS HOSP,DEPT MED BOSTON MA 02115 HARVARD UNIV,SCH MED,DEPT MED BOSTON MA 02115
Titolo Testata:
The American journal of physiology
fascicolo: 6, volume: 264, anno: 1993,
parte:, 2
pagine: 1896 - 1906
SICI:
0002-9513(1993)264:6<1896:ROCGIH>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT VENTRICULAR MYOCYTES; CHICK HEART-CELLS; NA-H EXCHANGE; INTRACELLULAR CALCIUM; METABOLIC INHIBITION; CONTRACTILE FAILURE; CA-2+; FLUORESCENCE; MECHANISMS; TRANSIENTS;
Keywords:
FURA-2; 2',7'-BIS(2-CARBOXYETHYL)-5(6)-CARBOXYFLUORESCEIN; ISCHEMIC INJURY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
M. Nishida et al., "ROLE OF CATION GRADIENTS IN HYPERCONTRACTURE OF MYOCYTES DURING SIMULATED ISCHEMIA AND REPERFUSION", The American journal of physiology, 264(6), 1993, pp. 1896-1906

Abstract

We examined the relationship between transsarcolemmal cation gradients and hypercontracture of cardiac myocytes in ischemia and reperfusionusing adult rat ventricular myocytes superfused with buffer mimickingnormal or ischemic extracellular fluid. Contractile performance of electrically stimulated cells was recorded by an optical video system simultaneously with measurements of intracellular Ca2+ concentration ([Ca2+]i) Using fura-2 or intracellular pH (pH(i)) using 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. While cells were exposed to simulated ischemia buffer, the transsarcolemmal H+ gradient was abolished, [Ca2+]i transient stopped, and twitch contraction of myocytes ceased. Upon reperfusion with normal buffer, H+ gradient was quickly restored, Ca2+ transients restarted with transient increase in systolic Ca2+, andtwitch contraction restarted with development of hypercontracture, which continued after [Ca2+]i returned to preischemic level even in the presence of near-normal concentrations of high-energy phosphates. Whenthe transsarcolemmal proton, Na+, and Ca2+ gradients were altered so that Na+ entry via Na+-H+ exchange and Ca2+ entry via Ca2+-Na+ exchange were made less favorable, the transient systolic overshoot of Ca2+ at reperfusion and development of hypercontracture was largely avoided. These results suggest that Na+ and then Ca2+ entry via the Na+-H+ andNa+-Ca2+ exchangers, respectively, probably contribute to the increase in [Ca2+]i and hypercontracture of myocytes at time of reperfusion in this model.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 09:40:18