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Titolo:
STAGE-DEPENDENT EXPRESSION OF MESSENGER-RNA FOR CYCLIC PROTEIN-2 DURING SPERMATOGENESIS IS MODULATED BY ELONGATE SPERMATIDS
Autore:
MAGUIRE SM; MILLAR MR; SHARPE RM; SAUNDERS PTK;
Indirizzi:
MRC,REPROD BIOL UNIT,37 CHALMERS ST EDINBURGH EH3 9EW MIDLOTHIAN SCOTLAND
Titolo Testata:
Molecular and cellular endocrinology
fascicolo: 1, volume: 94, anno: 1993,
pagine: 79 - 88
SICI:
0303-7207(1993)94:1<79:SEOMFC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
GERM-CELL TYPES; SERTOLI CELLS; CATHEPSIN-L; SEMINIFEROUS TUBULES; TESTICULAR FUNCTION; SECRETORY ACTIVITY; RAT TESTIS; ADULT-RAT; INVITRO; ACID;
Keywords:
CATHEPSIN-L; SERTOLI CELL; SPERMATOGENESIS; RAT TESTIS; ELONGATE SPERMATID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
S.M. Maguire et al., "STAGE-DEPENDENT EXPRESSION OF MESSENGER-RNA FOR CYCLIC PROTEIN-2 DURING SPERMATOGENESIS IS MODULATED BY ELONGATE SPERMATIDS", Molecular and cellular endocrinology, 94(1), 1993, pp. 79-88

Abstract

Cyclic protein 2 (CP-2) is a product of the Sertoli cell which is secreted in a cyclical manner according to the stage of the spermatogeniccycle. This study has assessed the influence of the germ cell complement on expression of CP-2 mRNA. Adult rats were treated with 650 mg/kgmethoxyacetic acid (MAA) to induce the specific depletion of > 80% ofpachytene and later spermatocytes from most tubules, and expression of CP-2 mRNA was then assessed at various times after treatment when particular germ cell types were depleted selectively. CP-2 mRNA was specifically localised to the Sertoli cells of the seminiferous tubules bynon-radioactive in situ hybridisation using a digoxigenin-labelled riboprobe. A stage specific variation in CP-2 mRNA levels was observed, with the mRNA being most abundant at stages IV-VII of the spermatogenic cycle. Northern analysis revealed that treatment with MAA led to an apparent increase in the amount of the major 1.7 kb CP-2 transcript when either pachytene spermatocytes or round spermatids were depleted. In contrast, the level of CP-2 mRNA was decreased by more than half at 21 days after MAA treatment. This decrease was confirmed by in situ hybridisation at 21 days after MAA treatment, when CP-2 mRNA expression was found to be decreased or absent from tubules at stages at which CP-2 mRNA is normally expressed (stages IV-VII) when elongate spermatidswere depleted selectively from these tubules. These observations leadus to hypothesise that elongate spermatids positively modulate CP-2 mRNA expression in the Sertoli cell.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:57:15