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Titolo:
INJURY TO CULTURED LIVER ENDOTHELIAL-CELLS DURING COLD PRESERVATION -ENERGY-DEPENDENT VERSUS ENERGY-DEFICIENCY INJURY
Autore:
RAUEN U; HINTZ K; HANSSEN M; LAUCHART W; BECKER HD; DEGROOT H;
Indirizzi:
HEINRICH HEINE UNIV,INST PHYSIOL CHEM 1,FORSCHERGRP LEBERSCHADIGUNG KLIN,MOORENSTR 5 W-4000 DUSSELDORF 1 GERMANY HEINRICH HEINE UNIV,INST PHYSIOL CHEM 1,FORSCHERGRP LEBERSCHADIGUNG KLIN,MOORENSTR 5 W-4000 DUSSELDORF 1 GERMANY
Titolo Testata:
Transplant international
fascicolo: 4, volume: 6, anno: 1993,
pagine: 218 - 222
SICI:
0934-0874(1993)6:4<218:ITCLED>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Keywords:
PRESERVATION, LIVER ENDOTHELIAL CELLS; LIVER ENDOTHELIAL CELLS, PRESERVATION; ENDOTHELIAL CELLS, LIVER, PRESERVATION; PRESERVATION SOLUTIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
NO
Recensione:
Indirizzi per estratti:
Citazione:
U. Rauen et al., "INJURY TO CULTURED LIVER ENDOTHELIAL-CELLS DURING COLD PRESERVATION -ENERGY-DEPENDENT VERSUS ENERGY-DEFICIENCY INJURY", Transplant international, 6(4), 1993, pp. 218-222

Abstract

Previously, we demonstrated an energy-dependent injury to cultured liver endothelial cells during cold incubation in University of Wisconsin (UW) solution. Here, the effects of Histidine-Tryptophan-Ketoglutarate (HTK) and Euro-Collins (EC) solutions on these cells were studied. In HTK solution, 83% +/- 4% of the cells had lost viability after 9 h of incubation at 4-degrees-C. The addition of cyanide (1 mM) to simulate hypoxic conditions protected the cells to the extent that only 9% +/- 1% of the cells lost viability over the same period; the addition of glucose (10 mM) led to increased cell injury. ATP levels were highest in the incubations with the most rapid loss of viability. In Krebs-Henseleit buffer and EC solution, in contrast, cell injury increased upon addition of cyanide; the addition of glucose to Krebs-Henseleit buffer decreased injury. We conclude that the injury to cultured liver endothelial cells during cold incubation in HTK solution is energy-dependent, as it is in UW solution, whereas cells behave differently in EC solution and Krebs-Henseleit buffer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 08:36:47