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Titolo:
EMULSIFYING AND OIL-BINDING PROPERTIES OF BOVINE SERUM-ALBUMIN AND ITS ENZYMATIC HYDROLYZATE
Autore:
SAITO M; CHIKUNI K; MONMA M; SHIMIZU M;
Indirizzi:
MINIST AGR FORESTRY & FISHERIES,NATL FOOD RES INST,2-1-2 KANNONDAI TSUKUBA IBARAKI 305 JAPAN UNIV SHIZUOKA,SCH FOOD & NUTR SCI SHIZUOKA 422 JAPAN
Titolo Testata:
Bioscience, biotechnology, and biochemistry
fascicolo: 6, volume: 57, anno: 1993,
pagine: 952 - 956
SICI:
0916-8451(1993)57:6<952:EAOPOB>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
LIMITED PROTEOLYSIS; PEPTIC HYDROLYZATE; PLASMA-PROTEIN; FRAGMENTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
M. Saito et al., "EMULSIFYING AND OIL-BINDING PROPERTIES OF BOVINE SERUM-ALBUMIN AND ITS ENZYMATIC HYDROLYZATE", Bioscience, biotechnology, and biochemistry, 57(6), 1993, pp. 952-956

Abstract

Bovine serum albumin (BSA) was hydrolyzed with proteases, and the emulsifying and oil-binding properties of the hydrolyzates were then studied. By limiting hydrolysis with trypsin, the emulsifying activity index (EAI) increased about 40% above its original level. In order to find out which peptides contributed to the good emulsifying properties ofthe hydrolyzate, the peptides adsorbed onto the emulsified oil globule surface were extracted and analyzed. SDS-polyacrylamide gel electrophoresis showed that a peptide with a molecular weight of about 24 kDa was preferentially adsorbed onto the oil surface. This 24 kDa peptide was found to be fairly hydrophobic and corresponds to the third domainof BSA, residues 377-582. In spite of the preferential adsorption characteristics of the 24 kDa peptide, this peptide itself had a lower EAI value than that of the whole hydrolyzate. The contribution of some small hydrophilic peptides, as well as the 24 kDa peptide, to the good emulsifying properties of the BSA hydrolyzate was suggested.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 02:49:29