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Titolo:
ALTERED PATTERN OF INSULIN-RECEPTOR ISOTYPES IN SKELETAL-MUSCLE MEMBRANES OF TYPE-2 (NON-INSULIN-DEPENDENT) DIABETIC SUBJECTS
Autore:
KELLERER M; SESTI G; SEFFER E; OBERMAIERKUSSER B; PONGRATZ DE; MOSTHAF L; HARING HU;
Indirizzi:
INST DIABET FORSCH MUNCHEN,KOLNER PL 1 W-8000 MUNICH 40 GERMANY INST DIABET FORSCH MUNCHEN,KOLNER PL 1 W-8000 MUNICH 40 GERMANY UNIV ROME,DIPARTIMENTO MED INTERNA I-00100 ROME ITALY FRIEDRICH BAUR INST MUNICH GERMANY
Titolo Testata:
Diabetologia
fascicolo: 7, volume: 36, anno: 1993,
pagine: 628 - 632
SICI:
0012-186X(1993)36:7<628:APOIII>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT FAT-CELLS; TRANSLOCATION; EXPRESSION; MELLITUS;
Keywords:
INSULIN RECEPTOR ISOTYPES; TYPE-2 (NON-INSULIN-DEPENDENT) DIABETES-MELLITUS; INSULIN RECEPTOR ANTIBODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
M. Kellerer et al., "ALTERED PATTERN OF INSULIN-RECEPTOR ISOTYPES IN SKELETAL-MUSCLE MEMBRANES OF TYPE-2 (NON-INSULIN-DEPENDENT) DIABETIC SUBJECTS", Diabetologia, 36(7), 1993, pp. 628-632

Abstract

The human insulin receptor exists in two isoforms (HIR-A alpha-subunit 719 amino acids and HIR-B alpha-subunit 731 amino acids) which are generated by alternative splicing of a small exon and display distinct patterns of tissue-specific expression. Using the polymerase chain reaction we have recently shown that skeletal muscle of non-diabetic individuals contains predominantly mRNA encoding HIR-A while in skeletal muscle derived from subjects with Type 2 (non-insulin-dependent) diabetes mellitus similar amounts of each mRNA are expressed. We used a polyclonal antibody which discriminates between HIR-A and HIR-B to assess the isoform expression at the protein level. The antibody showed clearly distinct displacement of insulin binding in skeletal muscle membranes of non-diabetic subjects compared to Type 2 diabetic subjects (displacement of specific I-125-insulin binding: 13 non-diabetic subjects 70.0 % +/- 14.34, 12 Type 2 diabetic subjects 32.6 % +/- 17.45). A control antibody which does not discriminate between both isoforms showed similar displacement of I-125-insulin in membranes of non-diabetic andType 2 diabetic subjects. These data suggest that the altered expression of receptor isotype mRNA in the skeletal muscle of Type 2 diabeticsubjects leads to an altered receptor isoform pattern in the plasma membrane. While skeletal muscle membranes of non-diabetic subjects contain predominantly HIR-A, membranes of Type 2 diabetic subjects show anincreased level of HIR-B in addition to HIR-A.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 11:46:33