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Titolo:
SYNTHESIS OF THERMAL PHASE-SEPARATING REACTIVE POLYMERS AND THEIR APPLICATIONS IN IMMOBILIZED ENZYMES
Autore:
LIU F; TAO GL; ZHUO RX;
Indirizzi:
WUHAN UNIV,DEPT CHEM WUHAN 430072 PEOPLES R CHINA
Titolo Testata:
Polymer Journal
fascicolo: 6, volume: 25, anno: 1993,
pagine: 561 - 567
SICI:
0032-3896(1993)25:6<561:SOTPRP>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Keywords:
POLYMERIZATION; PHASE SEPARATING REACTIVE POLYMERS; POLY(N-ISOPROPYLACRYLAMIDE); IMMOBILIZATION OF ENZYME; THERMOLYSIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
F. Liu et al., "SYNTHESIS OF THERMAL PHASE-SEPARATING REACTIVE POLYMERS AND THEIR APPLICATIONS IN IMMOBILIZED ENZYMES", Polymer Journal, 25(6), 1993, pp. 561-567

Abstract

This paper describes the synthesis of active ester copolymers from N-isopropylacrylamide (NIP) with N-acryloxysuccinimide (NAS), aminolysisof active esters, and determination of their average molecular weights. As a model, thermolysin was attached to the polymer to form soluble-insoluble immobilized enzymes capable of being centrifuged above their T(c), because poly(N-isopropylacylamide) (poly(NIP)) has a lower critical solution temperature (T(c)) of about 31-33-degrees-C. The effects of immobilization of enzymes have been studied including the amount of active ester, time, pH, temperature, and ratio of polymer to enzyme. The tests of thermal stability and repeated precipitation and separation showed that the use of T(c) as the technique of separation is possible.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/07/20 alle ore 19:13:52