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Titolo:
DEVELOPMENTS IN CELLULAR MICROCALORIMETRY WITH PARTICULAR EMPHASIS ONTHE VALUABLE ROLE OF THE ENERGY (ENTHALPY) BALANCE METHOD
Autore:
KEMP RB;
Indirizzi:
UNIV WALES,DEPT BIOL SCI,CELL BIOL LAB ABERYSTWYTH SY23 3DA DYFED WALES
Titolo Testata:
Thermochimica acta
, volume: 219, anno: 1993,
pagine: 17 - 41
SICI:
0040-6031(1993)219:<17:DICMWP>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATED RAT HEPATOCYTES; T-LYMPHOMA CELLS; HEAT-PRODUCTION; METABOLIC-ACTIVITY; MAMMALIAN-CELLS; TISSUE-CELLS; ANTINEOPLASTIC DRUGS; BLOOD-LYMPHOCYTES; MICRO-CALORIMETRY; SKELETAL-MUSCLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
104
Recensione:
Indirizzi per estratti:
Citazione:
R.B. Kemp, "DEVELOPMENTS IN CELLULAR MICROCALORIMETRY WITH PARTICULAR EMPHASIS ONTHE VALUABLE ROLE OF THE ENERGY (ENTHALPY) BALANCE METHOD", Thermochimica acta, 219, 1993, pp. 17-41

Abstract

This review of microcalorimetric studies of animal cells includes muscle and nerve as well as blood tissue, non-erythroid cells and transformed cells. It highlights the wide range of heat flow rate (phi = dQ/dt) data obtained for cells but points to their limited inherent value unless expressed per unit volume or biomass-scalar heat flux. Measurements of heat flow have been used in detecting clinical, pharmacological, toxicological and immunological changes-calorimetry as an analytical tool to assay metabolic activity in the face of pathological and xenobiosis-induced alterations. Microcalorimetry is a powerful and non-destructive technique of itself but its true strength is revealed when combined with other analytical procedures to allow access to the energy(enthalpy) balance method, which has its basis in the First Law of Thermodynamics. Its early use in dissecting all the chemical sources of heat in the thermogenesis of muscle is summarized, together with more recent studies of the metabolic burst in phagocytosis and catalytic pathways in cultured, anchorage-independent T-lymphoma and LS-L929 mousefibroblasts. The theoretical basis of the calorimetric-respirometric (CR) ratio is explained and its value in detecting anaerobic pathways in respiring cells is emphasized with reference to several cell types. Two ways of calculating ATP turnover (mumol infinity ATP h-1) are described, namely from heat flow and catabolic coupling flow; and there is opuscular reference to thermodynamic efficiency.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 10:55:18