Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
END-TO-END DIFFUSION-COEFFICIENTS AND DISTANCE DISTRIBUTIONS FROM FLUORESCENCE ENERGY-TRANSFER MEASUREMENTS - ENHANCED RESOLUTION BY USING MULTIPLE ACCEPTORS WITH DIFFERENT FORSTER DISTANCES
Autore:
MALIWAL BP; KUSBA J; WICZK W; JOHNSON ML; LAKOWICZ JR;
Indirizzi:
UNIV MARYLAND,SCH MED,DEPT BIOL CHEM,CTR FLUORESCENCE SPECT,108 N GREEN ST BALTIMORE MD 21201 UNIV MARYLAND,SCH MED,DEPT BIOL CHEM,CTR FLUORESCENCE SPECT,108 N GREEN ST BALTIMORE MD 21201 UNIV VIRGINIA,DEPT PHARMACOL CHARLOTTESVILLE VA 22908
Titolo Testata:
Biophysical chemistry
fascicolo: 3, volume: 46, anno: 1993,
pagine: 273 - 281
SICI:
0301-4622(1993)46:3<273:EDADDF>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
FREQUENCY-DOMAIN FLUOROMETRY; GLOBAL ANALYSIS; DECAY; DYNAMICS;
Keywords:
FORSTER DISTANCES; ENERGY TRANSFER; DIFFUSION; FREQUENCY-DOMAIN FLUORESCENCE; DONOR-ACCEPTOR TRANSFER; DISTANCE DISTRIBUTIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
B.P. Maliwal et al., "END-TO-END DIFFUSION-COEFFICIENTS AND DISTANCE DISTRIBUTIONS FROM FLUORESCENCE ENERGY-TRANSFER MEASUREMENTS - ENHANCED RESOLUTION BY USING MULTIPLE ACCEPTORS WITH DIFFERENT FORSTER DISTANCES", Biophysical chemistry, 46(3), 1993, pp. 273-281

Abstract

We measured distance distributions and end-to-end diffusion coefficients of donor-acceptor pairs linked by a flexible methylene chain usingfrequency-domain fluorescence energy transfer measurements. The donorwas an indole group, and two acceptors with different Forster distances were used. The uncertainties in the recovered parameters describingthe end-to-end distance distribution and diffusion coefficient were rather large when each donor-acceptor pair was analyzed separately. It was not possible to resolve distance distributions in the presence of intra-molecular diffusion when the Forster distance was comparable to the mean and half-width of the distribution. Global analysis using twoacceptors dramatically improved the resolution. Surprisingly, the Forster distances need not be very different, and a 20% difference between the two R0 values resulted in substantial improvements in resolution. Both the simulations and the experiments suggest the benefit of using global analysis with different Forster distances to obtain reliable distance distribution parameters in the presence of diffusion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 16:02:24