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Titolo:
ISOLATION AND CHARACTERIZATION OF THE RECEPTOR FOR VITELLOGENIN FROM FOLLICLES OF THE RAINBOW-TROUT, ONCORHYNCHUS-MYKISS
Autore:
TYLER CR; LANCASTER P;
Indirizzi:
BRUNEL UNIV,DEPT BIOL & BIOCHEM UXBRIDGE UB8 3PH MIDDX ENGLAND
Titolo Testata:
Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
fascicolo: 3, volume: 163, anno: 1993,
pagine: 225 - 233
SICI:
0174-1578(1993)163:3<225:IACOTR>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
SALMO-GAIRDNERI; OVARIAN FOLLICLES; CYPRINUS-CARPIO; OOCYTES; IDENTIFICATION; SOLUBILIZATION; SEQUESTRATION; PURIFICATION; PROTEINS; INVITRO;
Keywords:
VITELLOGENIN; RECEPTOR; FOLLICLE; RAINBOW TROUT, ONCORHYNCHUS-MYKISS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
C.R. Tyler e P. Lancaster, "ISOLATION AND CHARACTERIZATION OF THE RECEPTOR FOR VITELLOGENIN FROM FOLLICLES OF THE RAINBOW-TROUT, ONCORHYNCHUS-MYKISS", Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, 163(3), 1993, pp. 225-233

Abstract

The isolation and characterization of the receptor for vitellogenin from follicle membranes of the rainbow trout, Oncorhynchus mykiss, is described. Follicle membrane proteins subjected to SDS-polyacrylamide gel electrophoresis and subsequently to either protein staining or ligand blotting with radiolabelled vitellogenin (iodine-125-vitellogenin) demonstrated that the vitellogenin receptor has an apparent molecular mass of 200 kD (probably comprising of two 100-kD subunits) under non-reducing conditions. The vitellogenin binding sites were identified asspecific receptors: binding was saturable and the binding sites were both tissue specific to follicle membranes and exhibited ligand specificity. Scatchard analyses of specific binding data revealed a single class of binding sites with a high affinity for rainbow trout vitellogenin (K(d)=8.2.10(-9)mol.l-1). Both, brown trout, Salmo trutta, vitellogenin and carp, Cyprinus carpio, vitellogenin were able to displace the radiolabelled rainbow trout vitellogen from its receptor, although they were less effective than rainbow trout vitellogenin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:03:23