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Titolo:
A MODEL SYSTEM FOR A FLUOROMETRIC BIOSENSOR USING PERMEABILIZED ZYMOMONAS-MOBILIS OR ENZYMES WITH PROTEIN CONFINED DINUCLEOTIDES
Autore:
THORDSEN O; LEE SJ; DEGELAU A; SCHEPER T; LOOS H; REHR B; SAHM H;
Indirizzi:
UNIV MUNSTER,INST BIOCHEM,WILHELM KLEMM STR 2 W-4400 MUNSTER GERMANY UNIV MUNSTER,INST BIOCHEM,WILHELM KLEMM STR 2 W-4400 MUNSTER GERMANY UNIV HANNOVER,INST TECH CHEM W-3000 HANNOVER 1 GERMANY KFA JULICH GMBH,INST BIOTECHNOL 1 W-5170 JULICH 1 GERMANY
Titolo Testata:
Biotechnology and bioengineering
fascicolo: 3, volume: 42, anno: 1993,
pagine: 387 - 393
SICI:
0006-3592(1993)42:3<387:AMSFAF>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
SORBITOL PRODUCTION; ACID;
Keywords:
BIOSENSOR; GLUCOSE; FRUCTOSE; GLUCONOLACTONE; SORBITOL; ZYMOMONAS-MOBILIS; PERMEABILIZATION; NADPH; FLUORESCENCE; BIOPROCESS MONITORING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
12
Recensione:
Indirizzi per estratti:
Citazione:
O. Thordsen et al., "A MODEL SYSTEM FOR A FLUOROMETRIC BIOSENSOR USING PERMEABILIZED ZYMOMONAS-MOBILIS OR ENZYMES WITH PROTEIN CONFINED DINUCLEOTIDES", Biotechnology and bioengineering, 42(3), 1993, pp. 387-393

Abstract

Using permeabilized Zymomonas mobilis or glucose-fructose oxidoreductase isolated from this microorganism a model system for biosensors with a protein confined NADP(H) cofactor for the determination of glucose, fructose, gluconolactone, and sorbitol was developed. Either permeabilized microorganisms containing the oxidoreductase or the pure enzymewere confined via membrane separation in a small measuring chamber, that was integrated into a flow injection analysis system (FIA). The measuring principle was the monitoring of the NAD(P)H fluorescence, excited at 360 nm and measured at 450 nm. NADP(H), which is confined in the protein complex, was oxidized or reduced during the enzymatic reactions and the changes in the fluorescence intensity were related to the substrate concentration. The sensitivity of the system covered a rangefrom 0.001 to 100 g/L of the analyte depending on substrate and operating conditions. The applicability of this model system for bioprocessmonitoring was proved using samples from a Pseudomonas pseudoflava cultivation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 08:53:35