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Titolo:
PURIFICATION AND MOLECULAR-CLONING OF MOUSE RENAL DIPEPTIDASE
Autore:
SATOH S; KEIDA Y; KONTA Y; MAEDA M; MATSUMOTO Y; NIWA M; KOHSAKA M;
Indirizzi:
FUJISAWA PHARMACEUT CO LTD,DEPT BIOTECHNOL,PROD DEV LABS,1-6-2 CHOME,YODOGAWA KU OSAKA 532 JAPAN FUJISAWA PHARMACEUT CO LTD,DEPT BIOTECHNOL,PROD DEV LABS,1-6-2 CHOME,YODOGAWA KU OSAKA 532 JAPAN HIROSAKI UNIV,DEPT INTERNAL MED 2 HIROSAKI AOMORI 036 JAPAN
Titolo Testata:
Biochimica et biophysica acta
fascicolo: 3, volume: 1163, anno: 1993,
pagine: 234 - 242
SICI:
0006-3002(1993)1163:3<234:PAMOMR>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEHYDROPEPTIDASE-I; MICROSOMAL DIPEPTIDASE; CDNA CLONING; LEUKOTRIENE-D4; EXPRESSION; LUNG;
Keywords:
PURIFICATION; AMINO ACID SEQUENCE; DNA CLONING; RENAL DIPEPTIDASE; EXPRESSION; NORTHERN BLOTTING; (MOUSE);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
S. Satoh et al., "PURIFICATION AND MOLECULAR-CLONING OF MOUSE RENAL DIPEPTIDASE", Biochimica et biophysica acta, 1163(3), 1993, pp. 234-242

Abstract

Mouse renal dipeptidase (mouseRDP, EC 3.4.13.11) was purified from the membrane fraction of kidney. The molecular mass of the enzyme was 115 kDa by size-exclusion HPLC and SDS-PAGE under non-reduced conditionsand 58 kDa by SDS-PAGE under reduced conditions. The mouseRDP cDNA fragment was amplified from mouse kidney total RNA by reverse transcriptation-polymerase chain reaction (RT-PCR). The mouseRDP cDNA was isolated from a kidney cDNA library using the probe. The primary structure of mouseRDP deduced from the cDNA showed a high homology with renal dipeptidase from various mammals, except for the amino-terminal and carboxy-terminal domains. Recombinant mouseRDP obtained from transfected mouse L929 cells containing the expression plasmids has the same K(m) value and molecular mass as native mouse renal dipeptidase. From Northern blotting analysis, expression of the mouseRDP gene was recognized inboth kidney and liver.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 10:32:27