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Titolo:
A MODIFIED CERIUM-BASED HISTOCHEMICAL METHOD FOR DETECTION OF EXPERIMENTALLY-INDUCED ATPASE IMPAIRMENT IN GLOMERULI OF THE RAT-KIDNEY
Autore:
BALLER JFW; POELSTRA K; HARDONK MJ; BAKKER WW;
Indirizzi:
UNIV GRONINGEN,DEPT PATHOL,OOSTERSINGEL 63 9713 EZ GRONINGEN NETHERLANDS
Titolo Testata:
The Journal of histochemistry and cytochemistry
fascicolo: 7, volume: 41, anno: 1993,
pagine: 1105 - 1109
SICI:
0022-1554(1993)41:7<1105:AMCHMF>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOCHEMICAL DEMONSTRATION; ADENOSINE DIPHOSPHATASE; ANTITHROMBOTIC ACTIVITY; PHOSPHATASE-ACTIVITY; LOCALIZATION; MEMBRANE; SECTIONS;
Keywords:
GLOMERULAR ATPASE; CERIUM HISTOCHEMISTRY; PARAROSANILINE FIXATION; NUCLEOTIDASE STAINING;
Tipo documento:
Note
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
J.F.W. Baller et al., "A MODIFIED CERIUM-BASED HISTOCHEMICAL METHOD FOR DETECTION OF EXPERIMENTALLY-INDUCED ATPASE IMPAIRMENT IN GLOMERULI OF THE RAT-KIDNEY", The Journal of histochemistry and cytochemistry, 41(7), 1993, pp. 1105-1109

Abstract

We studied glomerular ATPase activity, as detectable at the light microscopic (LM) level in cryostat sections of the rat kidney, after unilateral local X-irradiation. The biochemically detectable reduction in glomerular ATPase activity after X-irradiation could be demonstrated at the LM level by application of a modified cerium-based technique. Results show a clear reduction of reaction product in glomeruli in X-irradiated kidneys as compared with the contralateral control kidney. Technical parameters (i.e., tissue fixation, section thickness, cerium concentration of the incubation mixture, and percentage H2O2 added for the amplification step) were established for optimal reproducibility ofthe staining results. We show that this modified staining protocol allows detection of differences of ATPase activity in contrast to conventional histochemical methods. Inhibition studies with various phosphatase inhibitors and competitive substrate inhibition experiments revealed that the enzyme is specific for nucleoside di- and triphosphatases. Since reduced glomerular adenine nucleotidase activity has recently been recognized as an early event in (experimental) glomerulonephritis,we feel that the new staining protocol presented here may be highly relevant for routine tissue section screening in nephropathological research.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 10:41:17