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Titolo:
IMPORTANCE OF THE NUCLEOTIDE LOOP MOIETY COORDINATED TO THE COBALT ATOM OF ADENOSYLCOBALAMIN FOR COENZYMIC FUNCTION IN THE DIOL DEHYDRASE REACTION
Autore:
ISHIDA A; ICHIKAWA M; KOBAYASHI K; HITOMI T; KOJIMA S; TORAYA T;
Indirizzi:
OKAYAMA UNIV,FAC ENGN,DEPT BIOTECHNOL OKAYAMA 700 JAPAN KYOTO UNIV,COLL LIBERAL ARTS & SCI,DEPT CHEM,SAKYO KU KYOTO 606 JAPAN MEIJI COLL ORIENTAL MED,DEPT CHEM HIYOSHI KYOTO 62903 JAPAN
Titolo Testata:
Journal of nutritional science and vitaminology
fascicolo: 2, volume: 39, anno: 1993,
pagine: 115 - 125
SICI:
0301-4800(1993)39:2<115:IOTNLM>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Keywords:
ADENOSYLCOBALAMIN; VITAMIN-B12 COENZYME; DIOL DEHYDRASE; ADENOSYLCOBINAMIDE; ADENOSYLCOBINAMIDE PHOSPHATE; N(3)-METHYLATED ADENOSYLCOBALAMIN; ALPHA-RIBAZOLE; ALPHA-RIBAZOLE-3'-PHOSPHATE; NUCLEOTIDE LOOP OF ADENOSYLCOBALAMIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
A. Ishida et al., "IMPORTANCE OF THE NUCLEOTIDE LOOP MOIETY COORDINATED TO THE COBALT ATOM OF ADENOSYLCOBALAMIN FOR COENZYMIC FUNCTION IN THE DIOL DEHYDRASE REACTION", Journal of nutritional science and vitaminology, 39(2), 1993, pp. 115-125

Abstract

Three analogs of adenosylcobalamin were synthesized and their coenzymic properties in the diol dehydrase reaction were studied. Neither adenosylcobinamide nor adenosylcobinamide phosphate was active as coenzyme and showed very low affinity for apoenzyme, irrespective of the presence of nucleotide loop fragments, such as 5,6-dimethylbenzimidazole, alpha-D-ribazole, or alpha-D-ribazole-3'-phosphate. The coordination of pyridine to the cobalt atom neither confers the coenzymic function upon adenosylcobinamide nor strengthens the inhibitory effect of cyanoaquacobinamide and methylcobinamide significantly. The analog of adenosylcobalamin in which the N-3 position of 5,6-dimethylbenzimidazole is methylated was also not active as coenzyme and showed very low affinity for apoenzyme. Since 3,5,6-trimethylbenzimidazole in this analog is no longer coordinated to the cobalt atom, these results show that at least a part of the nucleotide loop moiety coordinated to the cobalt atom of adenosylcobalamin is essential for tight binding to the apoenzyme and therefore for manifestation of coenzymic function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:22:09