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Titolo:
LOCALIZATION AND CONCENTRATION OF A NEW BIOACTIVE ACETIC SEMINAL FLUID PROTEIN (ASFP) IN BULLS (BOS-TAURUS)
Autore:
EINSPANIER R; AMSELGRUBER W; SINOWATZ F; HENLE T; ROPKE R; SCHAMS D;
Indirizzi:
TECH UNIV MUNICH,MILCH & LEBENSMITTEL WEIHENSTEPHAN FORSCHUNGSZENTRUM,INST PHYSIOL W-8050 FREISING GERMANY LMU,INST TIERANAT MUNICH GERMANY TECH UNIV MUNICH,LEHRSTUHL MILCHWISSENSCH W-8050 FREISING GERMANY
Titolo Testata:
Journal of Reproduction and Fertility
fascicolo: 1, volume: 98, anno: 1993,
pagine: 241 - 244
SICI:
0022-4251(1993)98:1<241:LACOAN>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
VESICLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
R. Einspanier et al., "LOCALIZATION AND CONCENTRATION OF A NEW BIOACTIVE ACETIC SEMINAL FLUID PROTEIN (ASFP) IN BULLS (BOS-TAURUS)", Journal of Reproduction and Fertility, 98(1), 1993, pp. 241-244

Abstract

The concentration of the new bioactive acetic seminal fluid protein (aSFP) was determined in body fluids of bulls. An aSFP-specific radioimmunoassay was elaborated and established for routine measurement. No crossreaction of the aSFP antibody was found with other seminal plasma proteins and known growth factors. The 14 kDa aSFP was detected in secretions of ampulla and seminal vesicles by immunoblot analysis, but not in testis, epididymis or blood. Large quantities of aSFP were measured by radioimmunoassay in the fluid from the ampulla (2.6 +/- 0.3 mg ml-1) and seminal vesicles (3.0 +/- 0.4 mg ml-1). Immunohistochemistry techniques demonstrated that aSFP was localized mainly in the secretory epithelium of ampulla and seminal vesicles. Large amounts of aSFP (4.0 +/- 0.4 mg ml-1) were present in seminal. plasma of bulls within a range of 1-7 mg ml-1, but aSFP could not be found on membranes of spermatozoa. Concentrations vary considerably among individuals, suggesting that aSFP may have a role in bovine reproduction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:13:50