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Titolo:
IN A CONCERTED ACTION KIT-LIGAND AND INTERLEUKIN-3 CONTROL THE SYNTHESIS OF SEROTONIN IN MURINE BONE MARROW-DERIVED MAST-CELLS - UP-REGULATION OF GTP CYCLOHYDROLASE-I AND TRYPTOPHAN 5-MONOOXYGENASE ACTIVITY BYTHE KIT-LIGAND
Autore:
ZIEGLER I; HULTNER L; EGGER D; KEMPKES B; MAILHAMMER R; GILLIS S; RODL W;
Indirizzi:
GSF MUNICH,INST KLIN MOLEK BIOL & TUMORGENET,MARCHIONINISTR 25 W-8000MUNICH 70 GERMANY GSF MUNICH,INST EXPTL HAMATOL W-8000 MUNICH 70 GERMANY IMMUNEX CORP SEATTLE WA 98101
Titolo Testata:
The Journal of biological chemistry
fascicolo: 17, volume: 268, anno: 1993,
pagine: 2544 - 2551
SICI:
0021-9258(1993)268:17<2544:IACAKA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-ENHANCING ACTIVITY; C-KIT; STEEL LOCUS; T-CELL; MOUSE; TETRAHYDROBIOPTERIN; PROLIFERATION; HYDROXYLASE; FORMS; 5-HYDROXYTRYPTAMINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
I. Ziegler et al., "IN A CONCERTED ACTION KIT-LIGAND AND INTERLEUKIN-3 CONTROL THE SYNTHESIS OF SEROTONIN IN MURINE BONE MARROW-DERIVED MAST-CELLS - UP-REGULATION OF GTP CYCLOHYDROLASE-I AND TRYPTOPHAN 5-MONOOXYGENASE ACTIVITY BYTHE KIT-LIGAND", The Journal of biological chemistry, 268(17), 1993, pp. 2544-2551

Abstract

Mouse bone marrow-derived mast cells (BMMC) store and release serotonin whose synthesis is initiated by tryptophan 5-monooxygenase. (6R)-H4biopterin serves as the natural cofactor for this reaction. GTP cyclohydrolase I catalyzes the first and rate-limiting step of its synthesis. In this study we demonstrate that among a panel of growth-promoting cytokines including kit ligand (KL), interleukin 3 (IL-3), IL-4, IL-9,and nerve growth factor, KL selectively enhances the synthesis of H4biopterin through up-regulation of GTP cyclohydrolase I activity to 6.2-fold levels. The activities of the subsequent enzymes 6-pyruvoyl-H4pterin synthase and sepiapterin reductase remain unaffected. The activity of tryptophan 5-monooxygenase was selectively enhanced 4.5-fold by the combination of IL-3 with KL. All other factors could not substitutefor KL. The constitutive high activity of aromatic L-amino acid decarboxylase is not different in cells cultured in IL-3 and/or KL. In consequence, the concerted action of IL-3 and KL on the GTP cyclohydrolaseI and the tryptophan 5-monooxygenase reaction enhances the productionof serotonin to about 20-fold levels. Additionally, KL specifically causes the release of about half of total serotonin produced. Hence, our data demonstrate a novel role of these cytokines for the function ofmouse BMMC and provide a coherent view of the regulation of serotoninsynthesis in this cell type.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 00:58:52