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Titolo:
CROSS-REACTIVITY BETWEEN POLLEN EXTRACTS FROM 6 ARTEMISIA SPECIES
Autore:
BRANDYS J; GRIMSOEN A; NILSEN BM; PAULSEN BS; PARK HS; HONG CS;
Indirizzi:
INST PHARM,DEPT PHARMACOGNOSY,POB 1068 BLINDERN N-0316 OSLO 3 NORWAY INST PHARM,DEPT PHARMACOGNOSY,POB 1068 BLINDERN N-0316 OSLO 3 NORWAY YONSEI UNIV,COLL MED,DEPT INTERNAL MED SEOUL SOUTH KOREA
Titolo Testata:
Planta medica
fascicolo: 3, volume: 59, anno: 1993,
pagine: 221 - 228
SICI:
0032-0943(1993)59:3<221:CBPEF6>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
MUGWORT POLLEN; VULGARIS L; GLYCOPROTEIN ALLERGEN; IDENTIFICATION; PURIFICATION; EMPHASIS;
Keywords:
ARTEMISIA SPP; POLLEN; ALLERGENS; CROSS-REACTIVITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
J. Brandys et al., "CROSS-REACTIVITY BETWEEN POLLEN EXTRACTS FROM 6 ARTEMISIA SPECIES", Planta medica, 59(3), 1993, pp. 221-228

Abstract

Pollen extracts of six different Artemisia species, A. vulgaris, A. scoparia, A. princeps, A. tridentata, A. annua, and A. campestris were compared using SDS-PAGE, IEF, immunoblotting, and immunoelectrophoretic methods. The band patterns obtained after SDS-PAGE and IEF showed a large degree of similarity between the extracts. Immunoblotting of these gels using a pool of sera from patients allergic to A. vulgaris gave essentially the same IgE-binding band pattern with all the extracts,demonstrating an extensive degree of cross-reactivity between A. vulgaris and the other Artemisia species. FRIE using a polyspecific antiserum against A. vulgaris showed that all the extracts contained severalantigens that were immunologically identical to antigens in A. vulgaris extract. Antigens showing immunological identity to the important A. vulgaris allergens Ag 12 and Art v II were present in all the extracts. The cross-reactivity between A. vulgaris and A. princeps was further verified by screening of ten Korean and nine Norwegian individual patient sera against extracts of both species in SDS-PAGE or IEF immunoblotting. Both groups of patients had essentially the same pattern of reactivity towards both pollen extracts.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 13:07:47