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Titolo:
ISOLATION AND AMINO-ACID-SEQUENCE OF A CHEMOTACTIC PROTEIN, LECT INTERLEUKIN-8, FROM A HUMAN MYELOID-LEUKEMIA CELL-LINE, ML-1
Autore:
SUZUKI K; YAMAKAWA Y; MATSUO Y; KAMIYA T; MINOWADA J; MIZUNO S;
Indirizzi:
NATL INST HLTH,DEPT BIOACT MOLEC,23-1 TOYAMA 1 CHOME,SHINJUKU KU TOKYO 162 JAPAN NATL INST HLTH,DEPT CELL BIOL & BIOCHEM TOKYO 162 JAPAN HAYASHIBARA BIOCHEM LABS INC,FUJISAKI CELL CTR OKAYAMA 402 JAPAN
Titolo Testata:
Immunology letters
fascicolo: 1, volume: 36, anno: 1993,
pagine: 71 - 82
SICI:
0165-2478(1993)36:1<71:IAAOAC>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEUTROPHIL-ACTIVATING PEPTIDE; CARCINOMA LU65C CELLS; POLYMORPHONUCLEAR LEUKOCYTES; MESSENGER-RNA; HYDROGEN-PEROXIDE; GENE-EXPRESSION; PURIFICATION; FIBROBLASTS; INDUCTION; INVITRO;
Keywords:
SEQUENCING; CHEMOTAXIS; LECT; INTERLEUKIN; MYELOID LEUKEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
K. Suzuki et al., "ISOLATION AND AMINO-ACID-SEQUENCE OF A CHEMOTACTIC PROTEIN, LECT INTERLEUKIN-8, FROM A HUMAN MYELOID-LEUKEMIA CELL-LINE, ML-1", Immunology letters, 36(1), 1993, pp. 71-82

Abstract

We looked for chemotaxin/interleukin 8 (CT/IL-8) activity in the culture fluids of 97 human, leukemia cell lines and found it in two of theT cell lines, six of the myeloid cell lines, and one of the normal B-cell lines. It was particularly strong in the culture fluids of two cell lines. These cell lines secreted a chemotactic protein into the culture fluids under certain conditions of stimulation with phorbol-12-myristate 13-acetate (PMA), lipopolysaccharide, or hemagglutinin-P. A myeloid leukemia cell line, ML-1, secreted an inducible chemotaxin when stimulated with PMA (1 ng/ml) for 24 h. We purified the chemotaxin from ML-1 cell culture fluid using an improved procedure: concentration with DEAE-Sepharose CL-6B and CM-Sepharose CL-6B, CM-Sepharose column chromatography, and reverse-phase 5TMS-300 column on HPLC with the retention time coinciding with that of LUCT/IL-8 [Suzuki et al., 1989, J. Exp. Med. 169, 1895]. The yield was 200 mug protein from 6 liters of the culture fluid. The N terminus of CT/IL-8 was AVLPRSAKELRXQXIKTYSK- - -, the same as that of LUCT/IL-8, which is constitutively secreted from lung giant cell carcinoma LU65C cells. The optimal concentration in the chemotactic activity of CT/IL-8, equivalent to that of bacterialchemotactic peptide fMet-Leu-Phe (10 nM), was found to be 5 nM. The results show that this chemotaxin is identical to LUCT/IL-8.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 06:18:48