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Titolo:
PURIFICATION AND CHARACTERIZATION OF THE CA-125 TUMOR-ASSOCIATED ANTIGEN FROM HUMAN ASCITES
Autore:
DELOSFRAILES MT; STARK S; JAEGER W; HOERAUF A; WILDT L;
Indirizzi:
UNIV ERLANGEN NURNBERG,FRAUENKLIN,DEPT OBSTET & GYNECOL W-8520 ERLANGEN GERMANY UNIV ERLANGEN NURNBERG,INST FRAUENHEILKUNDE W-8520 ERLANGEN GERMANY
Titolo Testata:
Tumor biology
fascicolo: 1, volume: 14, anno: 1993,
pagine: 18 - 29
SICI:
1010-4283(1993)14:1<18:PACOTC>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPITHELIAL OVARIAN CARCINOMAS; MONOCLONAL-ANTIBODY; IMMUNORADIOMETRIC ASSAY; MARKER CA-125; CANCER; CA125; CELLS; GLYCOPROTEIN; PROTEINS; MUCUS;
Keywords:
CA-125 ANTIGEN; AFFINITY PURIFICATION; IMMUNOBLOTTING; SDS-PAGE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
M.T. Delosfrailes et al., "PURIFICATION AND CHARACTERIZATION OF THE CA-125 TUMOR-ASSOCIATED ANTIGEN FROM HUMAN ASCITES", Tumor biology, 14(1), 1993, pp. 18-29

Abstract

CA 125 is an antigenic determinant associated with epithelial ovariancarcinomas, which- is recognized by a monoclonal antibody, OC 125. The biochemical structure, the immunological characteristics and the physiological function of CA 125 are unknown, principally because the molecule expressing it has not been purified to homogeneity. In the present study, we developed a single, one-step method for purifying CA 125 by column affinity chromatography, using the OC 125 antibody as immobilized ligand. The column proved to be highly specific for the purification of CA 125 from human ascites (HA). The antigen that eluted from the column has a specific activity of 6,240 +/- 120 U of CA 125/mg protein, the specific activity in the initial HA samples being 100 +/- 12 U/mg protein. The purified, immunoreactive CA 125 (IR-CA 125) was shown to be proteinaceous in nature. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and gel filtration characterization showed that the purified antigen exists as a high molecular weight (MW) complex, of up to 1. 5 million daltons, which could be dissociated under strong denaturing conditions, giving rise to moieties with an apparent MW of 205 and 55 kD. IR-CA 125 was also associated with a lower MW protein, with an apparent MW of 10-15 kD. The 205-kd MW protein was immunoreactive CA 125, as measured by immunoradiometric assay after being electroeluted from the polyacrylamide gel. Furthermore, when the affinity-purified antigen was subjected to SDS-PAGE, followed by immunoblotting, the lane which was reactive with the iodinated OC 125 antibody gave rise to a band with a molecular mass of 205 kD. Our results suggest that, on an analytical scale, the affinity column is useful for the purification of CA 125. The purified antigen is being used to investigate the possiblerole of CA 125 in the growth, development and physiological characteristics of human ovarian carcinomas in in vitro studies.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/12/20 alle ore 00:01:58