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Titolo:
HOMOLOGY OF A 130-KB REGION ENCLOSING THE ALPHA-GLOBIN GENE-CLUSTER, THE ALPHA-LOCUS CONTROLLING REGION, AND 2 NON-GLOBIN GENES IN HUMAN AND MOUSE
Autore:
KIELMAN MF; SMITS R; DEVI TS; FODDE R; BERNINI LF;
Indirizzi:
LEIDEN UNIV,DEPT HUMAN GENET,WASSENAARSEWEG 72 2333 AL LEIDEN NETHERLANDS
Titolo Testata:
Mammalian genome
fascicolo: 6, volume: 4, anno: 1993,
pagine: 314 - 323
SICI:
0938-8990(1993)4:6<314:HOA1RE>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSGENIC MICE; HUMAN GENOME; HIGH-LEVEL; REPLICATION; EXPRESSION; RECOMBINATION; CHROMOSOME-16; LOCALIZATION; CHROMATIN; SEQUENCES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
M.F. Kielman et al., "HOMOLOGY OF A 130-KB REGION ENCLOSING THE ALPHA-GLOBIN GENE-CLUSTER, THE ALPHA-LOCUS CONTROLLING REGION, AND 2 NON-GLOBIN GENES IN HUMAN AND MOUSE", Mammalian genome, 4(6), 1993, pp. 314-323

Abstract

The human alpha-globin gene cluster (30 kb) is embedded in a GC-rich isochore very close to the telomere of Chromosome (Chr) 16p. The alpha-Locus Controlling Region (alpha-LCR) is located upstream of the adultalpha-globin genes and has been shown to be essential for their expression. In this study we have been looking for expressed genes in the region upstream of the alpha-globin cluster to understand the role of the LCR-like element in the expression and replication timing of flanking gene clusters. We show that the upstream alpha-globin region is conserved over a 75-kb range and includes at least two oppositely transcribed non-globin genes, here referred to as Mid1 and Dist1. Complementary DNA sequences of 250 bp and 2.5 kb from Mid] (coordinate -68) and Dist] (coordinate -90 to -99), respectively, were isolated from human and mouse. The deduced partial amino acid sequences of these cDNAs are 81% and 95% identical for the Mid1 and Dist1 gene respectively. We have cloned a mouse cosmid ''contig'' which includes Dist1, Mid1, and theentire murine alpha-globin cluster. The murine homolog of the alpha-LCR was mapped upstream of the mouse globin genes at approximately the same position as in the human locus. Our results indicate that, in mouse and human, the alpha-globin loci and their flanking sequences are homologous over a range of at least 130 kb. The structural homology of this region in both mammals suggests also a functional one and indicates the mouse as a potential model for studying the role of the alpha-LCR controlling element in the regulation of expression and replicationtiming of the flanking gene clusters.

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Documento generato il 04/12/20 alle ore 20:05:49