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Titolo:
ISOLATION AND CHARACTERIZATION OF PHOSPHOLIPASE-A2 FROM AGKISTRODON-BILINEATUS (COMMON CANTIL) VENOM
Autore:
NIKAI T; KOMORI Y; YAGIHASHI S; OHARA A; OHIZUMI Y; SUGIHARA H;
Indirizzi:
MEIJO UNIV,FAC PHARM,DEPT MICROBIOL,TENPAKU KU,YAGOTOYAMA 150 NAGOYA 468 JAPAN MEIJO UNIV,FAC PHARM,DEPT MICROBIOL,TENPAKU KU,YAGOTOYAMA 150 NAGOYA 468 JAPAN NISSUI PHARMACEUT CO LTD,INST DIAGNOST REAGENTS YUKI IBARAKI 307 JAPAN TOHOKU UNIV,INST PHARMACEUT SENDAI MIYAGI 980 JAPAN
Titolo Testata:
International Journal of Biochemistry
fascicolo: 6, volume: 25, anno: 1993,
pagine: 879 - 884
SICI:
0020-711X(1993)25:6<879:IACOPF>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID-SEQUENCE; PURIFICATION; SNAKE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
T. Nikai et al., "ISOLATION AND CHARACTERIZATION OF PHOSPHOLIPASE-A2 FROM AGKISTRODON-BILINEATUS (COMMON CANTIL) VENOM", International Journal of Biochemistry, 25(6), 1993, pp. 879-884

Abstract

1. Phospholipase A, was isolated from Agkistrodon bilineatus venom bySephadex G-75 and CM-Cellulose column chromatographies. 2. The purified phospholipase A2-I gave a single band on disc polyacrylamide gel electrophoresis, isoelectric focusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis. 3. The enzyme preparation had a molecularweight of 14,000, isoelectric point of pH 8.77 and possessed 123 amino acid residues. 4. The purified phospholipase A2 possessed lethal, indirect hemolytic and anticoagulant activities. 5. The enzyme hydrolyzed the phospholipids phosphatidyl choline (PC), phosphatidyl ethanolamine (PE), phosphatidyl inositol (PI) and phosphatidyl) serine (PS). 6. The concentration of mouse diaphragm was inhibited and the contractionof guinea pig left atrium was increased by phospholipase A2-I. 7. Phospholipase A2 activity of this preparation was inhibited by ethylenediamine tetraacetic acid, p-bromo phenacyl bromide, n-bromo succinimide or dithiothreitol, but not by diisopropyl fluorophosphate or benzamidine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 13:17:57