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Titolo:
THE EFFECT OF AZATHIOPRINE, CYLOSPORINE-A AND INSULIN ON THE INVITRO LYMPHOCYTE-MEDIATED CYTOTOXICITY IN TYPE-I DIABETIC-PATIENTS
Autore:
HORVATH M; VARSANYI M; BANDI E; BALAZSI I; ROMICS L;
Indirizzi:
SEMMELWEIS UNIV MED,DEPT MED 3,1121 EOTVOS U 12 H-1085 BUDAPEST 8 HUNGARY JAHN FERENC MUNICIPAL HOSP,DEPT 1 BUDAPEST HUNGARY
Titolo Testata:
Experimental and clinical endocrinology
fascicolo: 3, volume: 100, anno: 1992,
pagine: 90 - 94
SICI:
0232-7384(1992)100:3<90:TEOACA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISLET-CELL ANTIBODIES; MELLITUS; IMMUNITY; PANCREAS; DISEASE; VIRUS; ONSET;
Keywords:
LYMPHOCYTE-MEDIATED CYTOTOXICITY (LMC); HUMAN PANCREAS-EXTRACT; AZATHIOPRINE; CYCLOSPORINE-A; INSULIN; ICA; HLA-DR POS T-LY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
M. Horvath et al., "THE EFFECT OF AZATHIOPRINE, CYLOSPORINE-A AND INSULIN ON THE INVITRO LYMPHOCYTE-MEDIATED CYTOTOXICITY IN TYPE-I DIABETIC-PATIENTS", Experimental and clinical endocrinology, 100(3), 1992, pp. 90-94

Abstract

Previously both specific and nonspecific immune reactions have been reported in patients with type I diabetes mellitus. In this study the effect of various immunosuppressive drugs and insulin was studied on invitro lymphocyte-mediated cytotoxicity in 20 type I diabetic patients. Twenty sex- and age-matched healthy subjects served as controls. Human pancreas-extract (300 mug/ml protein)-coated, 51-Chromium labeled chicken erythrocytes were used as target cells and separated T-lymphocytes as effector cells with and without azathioprine 50 mug/50 mul (Wellcome), Cyclosporine A 5 ng/50 mul (Sandoz) and MC Actrapid insulin 0.1 IU/50 mul (Novo). The degree of cytotoxicity was expressed with cytotoxic capacity: the number of maximal killed target cells. Simultaneously islet cell antibodies (ICA) in sera and the number of activated T-lymphocytes were assessed. Significant lymphocyte-mediated cytotoxicity was observed in die majority of type I diabetic patients (18/20), while no cytotoxicity was found in the control cases. The cytotoxicity decreased in all 16 patients using azathioprine or insulin, independently of ICA ans HLA-DR positivity. The number of killed target cells waslowered considerably by Cyclosporine A in all 18 patients having cytotoxicity against pancreas-extract. Our observations reveal that Cyclosporine A proved to be the most effective immunosuppressive agent in vitro. It decreases not only the leucocyte migration inhibition as previously observed, but also the lymphocyte-mediated cytotoxicity, which represents the late stage of cellular immune reactions against pancreatic tissue.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 14:57:38