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Titolo:
DETECTION OF CLOSTRIDIUM-DIFFICILE ENTEROTOXIN GENE IN CLINICAL SPECIMENS BY THE POLYMERASE CHAIN-REACTION
Autore:
BOONDEEKHUN HS; GURTLER V; ODD ML; WILSON VA; MAYALL BC;
Indirizzi:
HEIDELBERG REPATRIAT HOSP,DEPT MICROBIOL HEIDELBERG VIC 3081 AUSTRALIA HEIDELBERG REPATRIAT HOSP,DEPT MICROBIOL HEIDELBERG VIC 3081 AUSTRALIA
Titolo Testata:
Journal of Medical Microbiology
fascicolo: 5, volume: 38, anno: 1993,
pagine: 384 - 387
SICI:
0022-2615(1993)38:5<384:DOCEGI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIBIOTIC-ASSOCIATED COLITIS; TOXIN-A GENE; PSEUDOMEMBRANOUS COLITIS; REACTION AMPLIFICATION; IDENTIFICATION; PURIFICATION; DISEASE; ASSAY; DNA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
H.S. Boondeekhun et al., "DETECTION OF CLOSTRIDIUM-DIFFICILE ENTEROTOXIN GENE IN CLINICAL SPECIMENS BY THE POLYMERASE CHAIN-REACTION", Journal of Medical Microbiology, 38(5), 1993, pp. 384-387

Abstract

A rapid assay was developed for detection of the Clostridium difficile enterotoxin gene in stool specimens by means of the polymerase chainreaction (PCR). The PCR primers amplified a 63-bp repetitive sequenceof the enterotoxin gene, thereby generating a distinctive ladder pattern of DNA bands following electrophoresis. Crude DNA extracts from stools containing C. difficile produced one (63-bp) or more bands of thecharacteristic ladder. Of 172 stool specimens from 58 patients, 37 gave positive results by culture (15 specimens) or cytotoxin assay (36 specimens). When 36 available '' positive '' specimens were tested by the PCR assay, 34 (94%) gave positive results-24 by direct testing, and10 after extraction of DNA by the Qiagen procedure. Insufficient material of the remaining two specimens was available for DNA extraction. Of 21 stools '' negative '' for C. difficile by culture or cytotoxin assay, one gave a positive result by PCR and seven produced atypical bands. The rapid PCR detection technique for C. difficile was more sensitive than standard culture, and of a sensitivity similar to cytotoxin testing. The method has the potential for adoption in routine laboratory practice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 16:17:01