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Titolo:
FLOW CYTOMETRIC ANALYSIS OF THE CELL-CYCLE DISTRIBUTION OF SPLEEN LYMPHOCYTES ISOLATED FROM FISCHER-344 RATS EXPOSED TO ETHYL NITROSOUREA
Autore:
MORRIS SM; DOMON OE; MCGARRITY LJ; AIDOO A; KODELL RL; CASCIANO DA;
Indirizzi:
US FDA,NATL CTR TOXICOL RES,DIV GENET TOXICOL,HFT-120,1 NCTR DR JEFFERSON AR 72079
Titolo Testata:
Cell biology and toxicology
fascicolo: 1, volume: 9, anno: 1993,
pagine: 77 - 83
SICI:
0742-2091(1993)9:1<77:FCAOTC>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCTION; 2-MERCAPTOETHANOL; PROLIFERATION; MODULATION; DNA;
Keywords:
CELL-CYCLE ANALYSIS; COLCEMID; ENU; RAT LYMPHOCYTE DNA; FLOW CYTOMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
12
Recensione:
Indirizzi per estratti:
Citazione:
S.M. Morris et al., "FLOW CYTOMETRIC ANALYSIS OF THE CELL-CYCLE DISTRIBUTION OF SPLEEN LYMPHOCYTES ISOLATED FROM FISCHER-344 RATS EXPOSED TO ETHYL NITROSOUREA", Cell biology and toxicology, 9(1), 1993, pp. 77-83

Abstract

Current studies in our laboratory are designed to determine the frequency of genotoxic responses induced in lymphocytes isolated from Fischer 344 rats. To evaluate the effect of a model compound, N-ethyl-N-nitrosourea (ENU), on the cell-cycle distribution of spleen lymphocytes, 8-week old, female Fischer 344 rats were injected ip. with ENU and sacrificed 1, 2, 4, and 6 weeks after exposure. Four replicate cultures per dose per exposure period were established and cells were cultured for 66 hr. Colcemid, an agent which blocks cells in mitosis and inducesan accumulation of cells in the G2 + M peak, was added to two of the four cultures as a positive control. After a 3 hr incubation, the cells were harvested, the nuclei stained with propidium iodide, and the DNA content of the individual nuclei was quantified by flow cytometry. As expected, exposure to Colcemid resulted in an accumulation of cells in the G2 + M phase of the cell cycle, which was accompanied by a decrease in the G0 + G1 population. The increase in the G2 + M population was significant (p < 0.05) in cultures of lymphocytes assayed at 4 and6 weeks after exposure. The effect of increasing ENU concentration was an increase in the percentage of S-phase cells (p = 0.05) and a decrease (p < 0.02) in the percentage of G0 + G1 cells. This finding was observed only in those lymphocytes isolated 1 week after exposure. These findings indicate that flow cytometric analysis of the distribution of cells within the cell-cycle may provide insight into the effects oftoxicant exposure on mammalian cells.

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Documento generato il 05/04/20 alle ore 18:41:31