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Titolo:
EFFECTS OF THE IONOPHORE GRAMICIDIN-D ON ENERGY-METABOLISM IN HUMAN ERYTHROCYTES
Autore:
ENGSTROM I; WALDENSTROM A; RONQUIST G;
Indirizzi:
UNIV HOSP UPPSALA,DEPT MED S-75185 UPPSALA SWEDEN UNIV HOSP UPPSALA,DEPT INTERNAL MED S-75185 UPPSALA SWEDEN UNIV HOSP UPPSALA,DEPT CLIN CHEM S-75185 UPPSALA SWEDEN
Titolo Testata:
Scandinavian journal of clinical & laboratory investigation
fascicolo: 3, volume: 53, anno: 1993,
pagine: 247 - 252
SICI:
0036-5513(1993)53:3<247:EOTIGO>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
RED-CELL MEMBRANES;
Keywords:
ENERGY CHARGE; ADENINE-NUCLEOTIDES; MONOVALENT CATIONOPHORE; ERYTHROCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
10
Recensione:
Indirizzi per estratti:
Citazione:
I. Engstrom et al., "EFFECTS OF THE IONOPHORE GRAMICIDIN-D ON ENERGY-METABOLISM IN HUMAN ERYTHROCYTES", Scandinavian journal of clinical & laboratory investigation, 53(3), 1993, pp. 247-252

Abstract

Washed human erythrocytes were incubated in phosphate-buffered salineat 37-degrees-C with different concentrations (2-80 mg l-1) of the monovalent cationophore gramicidin D. The ionophore induced a decrease of ATP content and energy charge (EC) and a concomitant increase in ADPand AMP contents. At a concentration of 10 mg l-1 of the ionophore the reduction of EC and ATP concentration was about 50%. Increasing the concentration of the ionophore resulted in a substantial haemolysis with no further effect on the nucleotide levels. Lowering the concentration of the ionophore to 5 mg l-1 (to avoid haemolysis) lead to a similar effect on the energy status. Ouabain at 0.5 mmol l-1 in incubation medium distinctly blunted the ionophoric effect on EC and adenine nucleotides. Addition of glucose on the contrary reversed these effects byabout 80%. Hence, the glucose-induced increase in glycolytic flux as evidenced by an increase in lactate production was not sufficient to restore energy state.

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Documento generato il 30/11/20 alle ore 16:38:04