Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IONOPHORE A23187 REDUCES ENERGY-CHARGE BY ENHANCED ION PUMPING IN SUSPENDED HUMAN ERYTHROCYTES
Autore:
ENGSTROM I; WALDENSTROM A; RONQUIST G;
Indirizzi:
UNIV HOSP UPPSALA,DEPT MED S-75185 UPPSALA SWEDEN UNIV HOSP UPPSALA,DEPT INTERNAL MED S-75185 UPPSALA SWEDEN UNIV HOSP UPPSALA,DEPT CLIN CHEM S-75185 UPPSALA SWEDEN
Titolo Testata:
Scandinavian journal of clinical & laboratory investigation
fascicolo: 3, volume: 53, anno: 1993,
pagine: 239 - 246
SICI:
0036-5513(1993)53:3<239:IAREBE>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Keywords:
ENERGY CHARGE; ADENINE-NUCLEOTIDES; DIVALENT CATIONOPHORE; ERYTHROCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
I. Engstrom et al., "IONOPHORE A23187 REDUCES ENERGY-CHARGE BY ENHANCED ION PUMPING IN SUSPENDED HUMAN ERYTHROCYTES", Scandinavian journal of clinical & laboratory investigation, 53(3), 1993, pp. 239-246

Abstract

Washed human erythrocytes were basically incubated in phosphate-buffered saline at 37-degrees-C with different concentrations (1-5 mumol l-1) of the divalent cationophore A23187. This ionophore induced a decrease by about 75% of ATP content and energy charge (EC) and a concomitant increase in ADP and AMP contents in a dose-dependent fashion such that an inverse ATP/ADP relationship developed. EGTA at 1 mmol l-1 annihilated the effect of A23187 on energy status. When glucose was added to the basic incubation medium A23187 inclusion resulted in an elevated lactate production concomitantly with a partial restoration of EC. Introduction of Mg2+ to basic incubation medium containing glucose and the ionophore resulted in a sharp increase in lactate production with an energy state that was maintained at a control level. It is concluded that the low EC of erythrocytes obtained by this ionophore is the result of runaway ATPases dissociated from glycolysis. Since human erythrocytes are devoid of organelles other than the plasma membrane it is concluded that the ionophoric effect is exerted in the plasma membrane.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 20:11:52