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Titolo:
CAPACITATED AND ACROSOME REACTED SPERMATOZOA OF GOAT (CAPRA-INDICUS) - A FLUORESCENCE AND ELECTRON-MICROSCOPIC STUDY
Autore:
BAWA SR; PABST MA; WERNER G; BAINS HK;
Indirizzi:
PANJAB UNIV,DEPT BIOPHYS CHANDIGARH 160014 INDIA GRAZ UNIV,DEPT HISTOL & EMBRYOL A-8010 GRAZ AUSTRIA UNIV SAARLAND,FAC MED W-6650 HOMBURG GERMANY
Titolo Testata:
Andrologia
fascicolo: 3, volume: 25, anno: 1993,
pagine: 123 - 135
SICI:
0303-4569(1993)25:3<123:CAARSO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG SPERM; LECTIN-INDUCED AGGLUTINATION; INVITRO CAPACITATION; HAMSTER SPERMATOZOA; MOUSE SPERMATOZOA; RABBIT SPERMATOZOA; CUMULUS OOPHORUS; BOAR SPERMATOZOA; FREEZE-FRACTURE; SURFACE CHANGES;
Keywords:
SPERMATOZOA; CAPACITATION; ACROSOME REACTION; LECTINS; FREEZE-FRACTURE; GOAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
S.R. Bawa et al., "CAPACITATED AND ACROSOME REACTED SPERMATOZOA OF GOAT (CAPRA-INDICUS) - A FLUORESCENCE AND ELECTRON-MICROSCOPIC STUDY", Andrologia, 25(3), 1993, pp. 123-135

Abstract

Plasma membrane alterations accompanying in vitro capacitation and acrosome reaction of goat spermatozoa were studied using lectin labelling, scanning electron microscopy, and freeze-fracture methods. Fluorescein isothiocyanate linked lectins namely; Canavalia ensiformis (ConA),Maclura pomifera (MPA), Arachis hypogaea (PNA), Glycine max (SBA) andTriticum vulgaris (WGA) agglutinin were used to examine the distribution of surface carbohydrates during these two events. The head and thesperm tail reveal altered lectin labelling features after capacitation and acrosome reaction. After capacitation the surface coat components for MPA, SBA, and WGA are shed from the spermatozoon head. ConA receptors on the head are retained after capacitation but are partially shed in the acrosome reacted spermatozoa. SBA receptor sites appear on the sperm tail of the capacitated spermatozoa. Unusual morphological changes attending capacitation involve the sperm tail-end which developsa novel entity, which we have termed 'spatula'. The 'spatula' shows strong binding with ConA and WGA only. In the acrosome reacted spermatozoa the spatulated tail-end unwinds with a concomitant loss of lectin labelling. Highly ordered membrane particles, 'ladders' of the middle piece of the epididymal sperm tail, disappear and IMP clearings appearon the middle piece and in the spatulated ends of the capacitated spermatozoa. But in the acrosome reacted sperm IMPs reappear and are randomly disposed on the middle-piece and are clustered in small patches on the principal-piece. IMP free areas appear on the plasma membrane covering the acrosome and the outer acrosomal membrane (OAM) of the capacitated spermatozoa. The plasma membrane and OAM fuse at multiple fociand appear as acrosomal 'ghosts' which remain associated with the sperm head even after acrosome reaction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:17:52