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Titolo:
GROWTH-FACTORS MODULATE CLONAL GROWTH AND DIFFERENTIATION OF CULTUREDRABBIT LIMBAL AND CORNEAL EPITHELIUM
Autore:
KRUSE FE; TSENG SCG;
Indirizzi:
UNIV MIAMI,SCH MED,BASCOM PALMER EYE INST,DEPT OPHTHALMOL,POB 016880 MIAMI FL 33101 UNIV MIAMI,SCH MED,BASCOM PALMER EYE INST,DEPT OPHTHALMOL,POB 016880 MIAMI FL 33101
Titolo Testata:
Investigative ophthalmology & visual science
fascicolo: 6, volume: 34, anno: 1993,
pagine: 1963 - 1976
SICI:
0146-0404(1993)34:6<1963:GMCGAD>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERUM-FREE MEDIUM; NORMAL HUMAN KERATINOCYTES; EPIDERMAL GROWTH; STEM-CELLS; FACTOR-BETA; AUTOGRAFT TRANSPLANTATION; TERMINAL DIFFERENTIATION; INTEGRATED CONTROL; BOVINE BRAIN; FACTOR-ALPHA;
Keywords:
CORNEA; EPITHELIUM; GROWTH FACTORS; LIMBUS; STEM CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
71
Recensione:
Indirizzi per estratti:
Citazione:
F.E. Kruse e S.C.G. Tseng, "GROWTH-FACTORS MODULATE CLONAL GROWTH AND DIFFERENTIATION OF CULTUREDRABBIT LIMBAL AND CORNEAL EPITHELIUM", Investigative ophthalmology & visual science, 34(6), 1993, pp. 1963-1976

Abstract

Purpose. To compare the extent of modulation by various growth factors on clonal growth and differentiation between corneal and stem cell-containing limbal epithelium. Methods. A reported serum-free clonal growth assay was used. The mitogenic response was measured by colony forming efficiency, colony size, and bromodeoxyuridine labelling index; the differentiation was assessed by AE-5 monoclonal antibody staining. Results. As compared to controls in an insulin-containing basic medium,the addition of epidermal growth factor, acidic fibroblast growth factor, basic fibroblast growth factor, and a high dose of nerve growth factor was mitogenic for both epithelia. Cholera toxin was also mitogenic, but platelet-derived growth factor might not have, and insulin-like growth factor type I lacked an additive mitogenic effect with insulin. The mitogenic effect of epidermal growth factor differed from the others in its dose-dependent down-regulation and in having more migratory cells in epidermal growth factor-stimulated colonies. In contrast to these mitogens, transforming growth factor-beta 1 exhibited a clear inhibitory effect in the controls as well as in epidermal growth factor- or fibroblast growth factor-stimulated cultures. Transforming growth factor-beta I's inhibitory effect was correlated with strong AE-5 staining, whereas the mitogenic effect of epidermal growth factors, fibroblast growth factors or nerve growth factors was correlated with the presence of negative AE-5 colonies. Conclusion. There exist potential dynamic interactions among various growth-modifying cytokines in controlling epithelial growth and differentiation. The lack of specific activation of limbal stem cells in this culture assay also suggests that other unidentified factor(s) might be regulating stem cell functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 06:56:33