Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
BASE TRANSITIONS ARE THE MOST FREQUENT GENETIC CHANGES AT P53 IN GASTRIC-CANCER
Autore:
RENAULT B; VANDENBROEK M; FODDE R; WIJNEN J; PELLEGATA NS; AMADORI D; KHAN PM; RANZANI GN;
Indirizzi:
UNIV PAVIA,DEPT GENET & MICROBIOL,VIA ABBIATEGRASSO 207 I-27100 PAVIAITALY UNIV PAVIA,DEPT GENET & MICROBIOL,VIA ABBIATEGRASSO 207 I-27100 PAVIAITALY IST ONCOL ROMAGNOLO I-47100 FORLI ITALY MORGAGNI PIERANTONI HOSP I-47100 FORLI ITALY LEIDEN UNIV,SYLVIUS LAB,DEPT HUMAN GENET 2333 AL LEIDEN NETHERLANDS
Titolo Testata:
Cancer research
fascicolo: 11, volume: 53, anno: 1993,
pagine: 2614 - 2617
SICI:
0008-5472(1993)53:11<2614:BTATMF>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-LINES; GEL-ELECTROPHORESIS; MUTANT P53; MUTATIONS; POLYMORPHISM; CONFORMATION; CARCINOMAS; METASTASES; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
B. Renault et al., "BASE TRANSITIONS ARE THE MOST FREQUENT GENETIC CHANGES AT P53 IN GASTRIC-CANCER", Cancer research, 53(11), 1993, pp. 2614-2617

Abstract

We searched for P53 mutations in gastric carcinoma by analyzing tumorDNAs from 29 patients. We detected 13 different somatic mutations in 15 patients (52%) and a biallelic polymorphism in exon 6 (5 heterozygous subjects). The somatic mutations were mainly localized in the sequences corresponding to the highly conserved domains of the protein. Twelve samples showed a single base change: 11 missense and 1 nonsense mutations. Three samples showed deletions leading to a frame shift, to the in-frame loss of 2 amino acids, and to the deletion of a splicing site. All point mutations, except one, were transitions, and 91% of them were G:C-->A:T changes. We previously analyzed this panel of tumors for allelic loss at the 17p13 chromosomal region, where the P53 gene had previously been located: the results showed an increasing incidenceof allelic loss in late-stage tumors. On the contrary, in the presentstudy no trend between P53 mutations and tumor stages was found. Thisobservation indicates that mutation events precede allelic loss in gastric cancer. Half (54%) of the mutations occurred in samples without allelic loss, suggesting that specific mutated alleles, acting in a dominant negative fashion, can alter in vivo the P53 protein function.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 05:19:29