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Titolo:
HEPATOCYTE TRANSPLANTATION - AN ALTERNATIVE SYSTEM FOR EVALUATING CELL-SURVIVAL AND IMMUNOISOLATION
Autore:
GUPTA S; KIM SK; VEMURU RP; ARAGONA E; YERNENI PR; BURK RD; RHA CK;
Indirizzi:
YESHIVA UNIV ALBERT EINSTEIN COLL MED,MAR BESSIN LIVER RES CTR,1300 MORRIS PK AVE,ULLMAN 625 BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT MED BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT PEDIAT BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT MICROBIOL & IMMUNOL BRONX NY 10461 YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT OBSTET & GYNECOL BRONX NY 10461 MIT,BIOMAT SCI & ENGN LAB CAMBRIDGE MA 02139
Titolo Testata:
International journal of artificial organs
fascicolo: 3, volume: 16, anno: 1993,
pagine: 155 - 163
SICI:
0391-3988(1993)16:3<155:HT-AAS>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATITIS-B VIRUS; GENE-THERAPY; BIOARTIFICIAL PANCREAS; LIVER-FUNCTION; RAT; MICROENCAPSULATION; IMMUNOSUPPRESSION; EXPRESSION; REGION; HBSAG;
Keywords:
BIOARTIFICIAL LIVER; HEPATITIS-B VIRUS; HEPATOCYTE TRANSPLANTATION; IMMOBILIZED CELLS; TRANSGENIC MOUSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
S. Gupta et al., "HEPATOCYTE TRANSPLANTATION - AN ALTERNATIVE SYSTEM FOR EVALUATING CELL-SURVIVAL AND IMMUNOISOLATION", International journal of artificial organs, 16(3), 1993, pp. 155-163

Abstract

To evaluate systems for barrier immunoisolation of transplanted hepatocytes, we used transgenic mouse hepatocytes that secrete HBsAg. Hepatocytes were rapidly encapsulated in chitosan, a cationic polymer derived by deacetylation of chitin. Chitosan was allowed to electrostatically bond with anionic sodium alginate for creating an outer bipolymer membrane of the capsules. After encapsulation, hepatocyte viability remained unchanged for seven days in vitro with secretion of HBsAg into the culture medium throughout this period. Following intraperitoneal transplantation of encapsulated hepatocytes, HBsAg promptly appeared in blood of recipients. In congeneic recipients, serum HBsAg peaked at two weeks. Hepatocytes were present in recovered chitosan capsules and expressed HBsAg mRNA. In allogeneic recipients, however, serum HBsAg disappeared within one week and recovered chitosan capsules showed lymphomononuclear cells but not hepatocytes. Transplantation of chitosan encapsulatd HbsAg secreting hepatocytes failed to induce an anti-HBs response, suggesting modulation of the host immune response. These results indicate that transplantation systems using genetically modified hepatocytes which secrete gene products in the blood of recipients shouldfacilitate evaluation of hepatocyte encapsulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 06:44:17