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Titolo:
THE EFFECT OF TRANSFORMING GROWTH FACTOR-BETA(2)-SPECIFIC PHOSPHOROTHIOATE-ANTI-SENSE OLIGODEOXYNUCLEOTIDES IN REVERSING CELLULAR IMMUNOSUPPRESSION IN MALIGNANT GLIOMA
Autore:
JACHIMCZAK P; BOGDAHN U; SCHNEIDER J; BEHL C; MEIXENSBERGER J; APFEL R; DORRIES R; SCHLINGENSIEPEN KH; BRYSCH W;
Indirizzi:
UNIV WURZBURG,DEPT NEUROL,TUMORBIOL LAB,JOSEF SCHNEIDER STR 11 W-8700WURZBURG GERMANY UNIV WURZBURG,DEPT NEUROL,TUMORBIOL LAB,JOSEF SCHNEIDER STR 11 W-8700WURZBURG GERMANY UNIV WURZBURG,DEPT NEUROSURG W-8700 WURZBURG GERMANY INST VIROL & IMMUNOBIOL WURZBURG GERMANY MAX PLANCK INST BIOPHYS CHEM W-3400 GOTTINGEN GERMANY
Titolo Testata:
Journal of neurosurgery
fascicolo: 6, volume: 78, anno: 1993,
pagine: 944 - 951
SICI:
0022-3085(1993)78:6<944:TEOTGF>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-BETA; GLIOBLASTOMA CELLS; EXPRESSION; INHIBITION; INTERLEUKIN-1; LYMPHOCYTES; MODULATION; SUPPRESSION; ASTROCYTES; INVITRO;
Keywords:
TRANSFORMING GROWTH FACTOR-BETA; CELLULAR IMMUNOLOGY; CYTOKINE; MALIGNANT GLIOMA; PHOSPHOROTHIOATE-ANTI-SENSE OLIGODEOXYNUCLEOTIDE; INTERLEUKIN-2; LYMPHOKINE-ACTIVATED KILLER CELL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
41
Recensione:
Indirizzi per estratti:
Citazione:
P. Jachimczak et al., "THE EFFECT OF TRANSFORMING GROWTH FACTOR-BETA(2)-SPECIFIC PHOSPHOROTHIOATE-ANTI-SENSE OLIGODEOXYNUCLEOTIDES IN REVERSING CELLULAR IMMUNOSUPPRESSION IN MALIGNANT GLIOMA", Journal of neurosurgery, 78(6), 1993, pp. 944-951

Abstract

This in vitro study was aimed at restitution of transforming growth factor (TGF)-beta2-mediated suppression of T-lymphocyte activation within malignant gliomas. In early-passage tumor cell cultures of two glioblastomas (HTZ-153 and HTZ-209) and one malignant astrocytoma classified as World Health Organization Grade III (HTZ-243), autologous peripheral blood mononuclear cells were activated by interleukin-1alpha and interleukin-2 in vitro (lymphokine-actived killer cells) and tested for cytotoxic and proliferative activity. In expression studies (Westernblot and Northern hybridization) of all three tumors, TGF-beta could be detected at the protein and messenger ribonucleic acid (mRNA) levels. A polyclonal anti-TGF-beta neutralizing antibody did not enhance lymphocyte proliferation upon stimulation with tumor targets (H-3-thymidine incorporation) and slightly stimulated lymphocyte cytotoxicity against autologous target cells. Preincubation of target cells for 12 hours with TGF-beta2-specific phosphorothioate-anti-sense oligodeoxynucleotides (S-ODN's) did. however, enhance lymphocyte proliferation up to 2.5-fold and autologous tumor cytotoxicity up to 60%, compared to controls not treated with S-ODN's. Incubation of tumor cells with TGF-beta2-specific S-ODN's resulted in decreased TGF-beta-specific immunoreactivity in cultured glioma cells, in reduced TGF-beta2 protein concentration (Western blot), and in a change in the expression pattern of TGF-beta2 mRNA's. These observations may have implications for in vivo andin vitro activation of a cellular immune response against autologous malignant glioma cells.

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Documento generato il 29/09/20 alle ore 09:27:17