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Titolo:
GONADOTROPIN-MEDIATED INHIBITION OF PROTEOLYTIC-ENZYMES PRODUCED BY HUMAN TROPHOBLAST IN CULTURE
Autore:
MILWIDSKY A; FINCIYEHESKEL Z; YAGEL S; MAYER M;
Indirizzi:
HADASSAH HOSP MT SCOPUS,DEPT CLIN BIOCHEM,POB 24035 IL-91240 JERUSALEM ISRAEL HADASSAH HOSP MT SCOPUS,DEPT CLIN BIOCHEM,POB 24035 IL-91240 JERUSALEM ISRAEL HADASSAH HEBREW UNIV HOSP,DEPT OBSTET IL-91240 JERUSALEM ISRAEL HADASSAH HEBREW UNIV HOSP,DEPT GYNECOL IL-91240 JERUSALEM ISRAEL HADASSAH HEBREW UNIV,SCH MED IL-91240 JERUSALEM ISRAEL
Titolo Testata:
The Journal of clinical endocrinology and metabolism
fascicolo: 5, volume: 76, anno: 1993,
pagine: 1101 - 1105
SICI:
0021-972X(1993)76:5<1101:GIOPPB>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMINOGEN-ACTIVATOR PRODUCTION; CELLS-INVITRO; EXTRACELLULAR-MATRIX; PROTEASE INHIBITORS; MALIGNANT-CELLS; DEGRADATION; CYTOTROPHOBLASTS; DIFFERENTIATION; IMPLANTATION; LOCALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
A. Milwidsky et al., "GONADOTROPIN-MEDIATED INHIBITION OF PROTEOLYTIC-ENZYMES PRODUCED BY HUMAN TROPHOBLAST IN CULTURE", The Journal of clinical endocrinology and metabolism, 76(5), 1993, pp. 1101-1105

Abstract

We investigated the effect of gonadotropins on proteases that were suggested to be implicated in the invasive activity of the trophoblast. hCG levels ranging from 10 X 10(3) to 333 x 10(3) IU/L produced a dose-dependent inhibition of the in vitro globinolytic activity of the purified proteases trypsin, chymotrypsin, and urokinase, but failed to inhibit plasmin, collagenase, elastase, and tissue-type plasminogen activator. Likewise, FSH inhibited purified trypsin and urokinase, but notplasmin or tissue-type plasminogen activator. Culture medium conditioned with human trophoblast displayed serine protease and urokinase-like activities; exposure of the cultured trophoblast to exogenous hCG markedly suppressed serine protease and urokinase activities in the conditioned medium. A short treatment of the conditioned medium with trypsin abolished the hCG-mediated inhibition of urokinase activity. The present findings offer an explanation for earlier observations that hCG reduced collagenase activity in trophoblasts without affecting the level of collagenase-specific mRNA. The present results are also consistent with the concept that hCG, by its direct ability to inhibit certainserine proteases and urokinase in trophoblast, suppresses a protease-mediated conversion of procollagenase to active collagenase. The ability of hCG to prevent initiation of the collagenolytic cascade suggeststhat gonadotropins may regulate the transient invasive activity of the trophoblast.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 09:33:34