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Titolo:
THE DISTRIBUTION OF INTRACELLULAR CALCIUM CHELATOR (FURA-2) IN A POPULATION OF INTACT HUMAN RED-CELLS
Autore:
LEW VL; ETZION Z; BOOKCHIN RM; DACOSTA R; VAANANEN H; SASSAROLI M; EISINGER J;
Indirizzi:
UNIV CAMBRIDGE,PHYSIOL LAB,DOWNING ST CAMBRIDGE CB2 3EG ENGLAND ALBERT EINSTEIN COLL MED NEW YORK NY 00000 CUNY MT SINAI SCH MED,DEPT PHYSIOL & BIOPHYS NEW YORK NY 10029
Titolo Testata:
Biochimica et biophysica acta
fascicolo: 1, volume: 1148, anno: 1993,
pagine: 152 - 156
SICI:
0006-3002(1993)1148:1<152:TDOICC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
FORMALDEHYDE; POTASSIUM; ANEMIA; ESTERS;
Keywords:
FURA-2; CYTOMETRY; CALCIUM CHELATOR; (ERYTHROCYTE);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
15
Recensione:
Indirizzi per estratti:
Citazione:
V.L. Lew et al., "THE DISTRIBUTION OF INTRACELLULAR CALCIUM CHELATOR (FURA-2) IN A POPULATION OF INTACT HUMAN RED-CELLS", Biochimica et biophysica acta, 1148(1), 1993, pp. 152-156

Abstract

Using quantitative fluorescence microscopy of red cells loaded non-disruptively with 1-2.5 mmol/l cells of fura-2, we examined the distribution of the incorporated free chelator among and within individual cells. Cytoplasmic hemoglobin quenched the effective fluorescence yield of fura-2 by a factor of about 100. All red cells were found to fluoresce upon excitation at 380 nm, and the fluorescence intensities they emitted at 510 nm were approximately +/- 20% about the mean intensity, indicating a fairly uniform distribution of incorporated chelator amongthe cells. Red cells loaded with these high levels of fura-2 retainedtheir biconcave shape, and a comparison between their transmission images at 415 nm and their fura-2 fluorescence images suggests that the concentration of fura-2 was also uniform throughout the cytosol. Theseresults validate assumptions made in earlier experiments with non-fluorescent incorporated Ca2+ chelators, and demonstrate the feasibility of fura-2 and Ca2+ imaging of intact red cells, despite considerable quenching of probe fluorescence by hemoglobin.

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Documento generato il 19/09/20 alle ore 09:16:32