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Titolo:
CHARACTERIZATION OF A NEW CLASS OF OLEOSINS SUGGESTS A MALE GAMETOPHYTE-SPECIFIC LIPID STORAGE PATHWAY
Autore:
ROBERTS MR; HODGE R; ROSS JHE; SORENSEN A; MURPHY DJ; DRAPER J; SCOTT R;
Indirizzi:
UNIV LEICESTER,DEPT BOT,UNIV RD LEICESTER LE1 7RH ENGLAND UNIV LEICESTER,DEPT BOT,UNIV RD LEICESTER LE1 7RH ENGLAND JOHN INNES CTR PLANT SCI RES,CAMBRIDGE LAB,DEPT BRASS & OILSEEDS RES NORWICH NR4 7UJ ENGLAND
Titolo Testata:
Plant journal
fascicolo: 5, volume: 3, anno: 1993,
pagine: 629 - 636
SICI:
0960-7412(1993)3:5<629:COANCO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
BODY MEMBRANE-PROTEIN; BRASSICA-NAPUS; OIL BODIES; RAPESEED; SEED; SEQUENCES; SURFACE; POLLEN; PLANTS; MAIZE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
M.R. Roberts et al., "CHARACTERIZATION OF A NEW CLASS OF OLEOSINS SUGGESTS A MALE GAMETOPHYTE-SPECIFIC LIPID STORAGE PATHWAY", Plant journal, 3(5), 1993, pp. 629-636

Abstract

The expression and sequences of two related anther-specific cDNAs (13and C98) of Brassica napus (oilseed rape) were examined. These cDNAs were found to exhibit significant predicted amino acid sequence similarity with a group of seed-specific proteins, the oleosins, which are involved in oil body membrane structure. Pollen of B. napus also contains oil bodies, and the synthesis and accumulation of these organelles correlates with expression of the 13 and C98 transcripts. The protein content of purified pollen oil bodies was therefore examined in order to determine the presence or absence of possible oleosins. One major protein species of 14 kDa was identified and subjected to N-terminal amino acid sequence determination. The sequence of this pollen oil body associated protein is homologous to the predicted sequence of one of the anther-specific cDNAs, 13, implying that this cDNA represents an anther-specific oleosin gene expressed in developing pollen. The expression of other genes active during seed oil body formation was also examined, and these were found to be specific to the sporophyte, inferringthe existence of specific components of the pathway of lipid synthesis and storage in the male gametophyte. These possibilities and the relationship between different oleosin genes are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 01:29:17