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Titolo:
SYNCHRONOUS CALCIUM OSCILLATIONS IN CEREBELLAR GRANULE CELLS IN CULTURE MEDIATED BY NMDA RECEPTORS
Autore:
LAWRIE AM; GRAHAM ME; THORN P; GALLACHER DV; BURGOYNE RD;
Indirizzi:
UNIV LIVERPOOL,PHYSIOL LAB,POB 147 LIVERPOOL L69 3BX ENGLAND UNIV LIVERPOOL,PHYSIOL LAB,POB 147 LIVERPOOL L69 3BX ENGLAND
Titolo Testata:
NeuroReport
fascicolo: 5, volume: 4, anno: 1993,
pagine: 539 - 542
SICI:
0959-4965(1993)4:5<539:SCOICG>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SURVIVAL; RAT; STIMULATION; NEURONS; ACID; DIFFERENTIATION; DEPOLARIZATION;
Keywords:
N-METHYL-D-ASPARTATE; EXCITATORY AMINO ACIDS; CALCIUM; CEREBELLUM; GRANULE CELLS; OSCILLATIONS; NEURONAL DIFFERENTIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
A.M. Lawrie et al., "SYNCHRONOUS CALCIUM OSCILLATIONS IN CEREBELLAR GRANULE CELLS IN CULTURE MEDIATED BY NMDA RECEPTORS", NeuroReport, 4(5), 1993, pp. 539-542

Abstract

THE concentration of cytosolic Ca2+ ([Ca2+]i) was monitored in cerebellar granule cell cultures by digital imaging of fura-2 loaded cells. In the presence of Mg2+, cells grown in low K+ cultures responded to N-methyl-D-aspartate (NMDA) with uniform increases in [Ca2+]i from a stable basal [Ca2+]i. In contrast, in Mg2+-free medium, low K+ cultures showed spontaneous, synchronized [Ca2+]i oscillations from 4 days in culture. The oscillations were rapidly blocked by Mg2+, D,L-2-amino-5-phosphonovalerate, or tetrodotoxin. The development of oscillatory behaviour depended on the culture conditions and was not observed in cultures grown in high K+. These data show a high degree of connectivity established within 4 days in culture by dissociated granule cells allowing synchronized activity mediated through synaptic mechanisms.

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Documento generato il 13/07/20 alle ore 10:58:05