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Titolo:
MCP-1 EXPRESSION BY RAT TYPE-II ALVEOLAR EPITHELIAL-CELLS IN PRIMARY CULTURE
Autore:
PAINE R; ROLFE MW; STANDIFORD TJ; BURDICK MD; ROLLINS BJ; STRIETER RM;
Indirizzi:
UNIV MICHIGAN,DEPT INTERNAL MED,DIV PULM & CRIT CARE MED,3916 TAUBMANCTR,BOX 0360 ANN ARBOR MI 48109 HARVARD UNIV,DANA FARBER CANC INST CAMBRIDGE MA 02138
Titolo Testata:
The Journal of immunology
fascicolo: 10, volume: 150, anno: 1993,
pagine: 4561 - 4570
SICI:
0022-1767(1993)150:10<4561:MEBRTA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCYTE CHEMOATTRACTANT PROTEIN-1; BLOOD MONONUCLEAR LEUKOCYTES; AMINO-ACID ANALYSIS; GENE-EXPRESSION; HUMAN-FIBROBLASTS; MACROPHAGES; CLONING; LUNG; PURIFICATION; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
R. Paine et al., "MCP-1 EXPRESSION BY RAT TYPE-II ALVEOLAR EPITHELIAL-CELLS IN PRIMARY CULTURE", The Journal of immunology, 150(10), 1993, pp. 4561-4570

Abstract

Recruitment and activation of mononuclear phagocytes are potentially critical regulatory events for control of pulmonary inflammation. Located at the boundary between the alveolar airspace and the interstitium, alveolar epithelial cells are ideally situated to regulate the recruitment and activation of mononuclear phagocytes through the productionof cytokines in response to inflammatory stimulation from the alveolar space. To test this hypothesis, we investigated the production of monocyte chemotactic polypeptide-1 (MCP-1), a protein that is chemotactic for and that activates monocytes, by rat type II alveolar epithelialcells in primary culture. Immunocytochemical staining using anti-murine JE, an antibody recognizing rat MCP-1, demonstrated cell-associatedMCP-1 Ag throughout the monolayer. The intensity of staining was increased in response to IL-1 beta. When type II epithelial cells formed atight monolayer on a filter support, there was polar secretion of MCP-1 Ag into the apical compartment by both control and IL-1-stimulated cells as measured by specific MCP-1 ELISA. Northern blot analysis revealed that IL-1 and TNF-alpha stimulated MCP-1 mRNA expression in a dose-dependent manner, whereas dexamethasone blocked MCP-1 expression by cells stimulated with IL-1. In contrast to previous results using transformed epithelial cell lines, MCP-1 mRNA was induced in these primarycultures directly by stimulation with LPS. These data suggest that alveolar epithelial cells may have an important and previously unrecognized role in the initiation and maintenance of inflammatory processes in the lung by recruiting and activating circulating monocytes through the production of MCP-1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 14:42:38