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Titolo:
CYTOTOXIC T-LYMPHOCYTE CONTROL DURING ECTROMELIA (MOUSEPOX) VIRUS-INFECTION - INTERACTION BETWEEN MHC-RESTRICTED CELLS ANALYZED BY NONRADIOACTIVE FLUOROMETRY
Autore:
TOKA FN; NIEMIALTOWSKI MG; DEFAUNDEZ IS; GIERYNSKA M;
Indirizzi:
AGR UNIV WARSAW,FAC VET MED,DEPT MICROBIOL & IMMUNOL,GROCHOWSKA 272 PL-03849 WARSAW POLAND AGR UNIV WARSAW,FAC VET MED,DEPT MICROBIOL & IMMUNOL PL-03849 WARSAW POLAND
Titolo Testata:
Acta virologica
fascicolo: 5-6, volume: 40, anno: 1996,
pagine: 239 - 244
SICI:
0001-723X(1996)40:5-6<239:CTCDE(>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
HERPES-SIMPLEX VIRUS; MEDIATED CYTOTOXICITY; IMMUNE-RESPONSE; CYTO-TOXICITY; IN-VITRO; MICE; ASSAY; CD8+; CD4+; RESISTANCE;
Keywords:
ECTROMELIA VIRUS; CYTOTOXIC T LYMPHOCYTES; FLUOROMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
F.N. Toka et al., "CYTOTOXIC T-LYMPHOCYTE CONTROL DURING ECTROMELIA (MOUSEPOX) VIRUS-INFECTION - INTERACTION BETWEEN MHC-RESTRICTED CELLS ANALYZED BY NONRADIOACTIVE FLUOROMETRY", Acta virologica, 40(5-6), 1996, pp. 239-244

Abstract

Cytotoxic T lymphocyte (CTL) activity of draining lymph node (DLN) cells isolated from BALB/c mice infected with ectromelia virus (EV) was examined using a fluorometric cell-mediated cytotoxicity (CMC) assay. Specific lysis of target cells A20 and EMT-6 primed with EV was demonstrated. The classical CD8(+) cytolytic pathway dominated (72.7%) as compared to that of CD4(+) (27.3%) in the cellular response during acuteEV infection. Also an alternative method for determining CMC, employing a bisbenzamide dye for labelling target cells, is described. Coefficient variations of relative fluorescence were below 6%, that makes the method sensitive and reliable.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 09:25:30