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Titolo:
PURIFICATION AND PROPERTIES OF CLOSTEROVIRUS-LIKE PARTICLES ASSOCIATED WITH A WHITEFLY-TRANSMITTED DISEASE OF SWEET-POTATO
Autore:
COHEN J; FRANCK A; VETTEN HJ; LESEMANN DE; LOEBENSTEIN G;
Indirizzi:
AGR RES ORG,VOLCANI CTR,DEPT VIROL IL-50250 BET DAGAN ISRAEL AGR RES ORG,VOLCANI CTR,DEPT VIROL IL-50250 BET DAGAN ISRAEL BIOL BUNDESANSTALT LAND & FORSTWIRTSCHAFT,INST BIOCHEM & PLANT VIROL BRAUNSCHWEIG GERMANY
Titolo Testata:
Annals of Applied Biology
fascicolo: 2, volume: 121, anno: 1992,
pagine: 257 - 268
SICI:
0003-4746(1992)121:2<257:PAPOCP>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
DOUBLE-STRANDED-RNA; CUCUMBER MOSAIC-VIRUS; BEET YELLOWS VIRUS; POLYACRYLAMIDE GELS; NUCLEOTIDE-SEQUENCE; PROTEIN; MOTTLE; PLANT;
Keywords:
SWEET POTATO WHITEFLY TRANSMITTED VIRUS; CLOSTEROVIRUS; VESICLES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
J. Cohen et al., "PURIFICATION AND PROPERTIES OF CLOSTEROVIRUS-LIKE PARTICLES ASSOCIATED WITH A WHITEFLY-TRANSMITTED DISEASE OF SWEET-POTATO", Annals of Applied Biology, 121(2), 1992, pp. 257-268

Abstract

A virus causing sunken veins on 'Georgia Jet' sweet potato, and yellow brittle leaves and stunting on Ipomoea setosa, was purified and a specific antiserum was prepared. Flexuous particles with a normal lengthof 850 nm and a diameter of 12 nm with an open helical structure typical of closteroviruses were observed. The virus particle protein has an apparent mol. wt of c. 34 kD. Double-stranded RNA isolated from SPSVV-infected I. setosa and subjected to electrophoresis in agarose consisted of one major band with an estimated M(r) of 10.5 kbp and two minor bands with M(r) of 9.0 and 5.0 kbp. Fibril-containing vesicles in phloem cells were observed in ultrathin sections of infected leaf tissues. The virus was transmitted by the whitefly Bemisia tabaci in a semi-persistent manner and by grafting, but not mechanically. The virus could be transmitted to various Ipomoea species, to Nicotiana clevelandii, N. benthamiana and Amaranthus palmeri. The virus did not react with an antiserum to lettuce infectious yellows virus. Based on particle morphology, serology and symptom expression, the virus appears unique and different from all other reported whitefly-transmitted closteroviruses. We propose it be named ''sweet potato sunken vein virus'' (SPSVV).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 14:41:14