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Titolo:
EFFECTS OF HUMAN PAPILLOMAVIRUS TYPE-18-SPECIFIC ANTISENSE OLIGONUCLEOTIDES ON THE TRANSFORMED PHENOTYPE OF HUMAN CARCINOMA CELL-LINES
Autore:
STEELE C; COWSERT LM; SHILLITOE EJ;
Indirizzi:
UNIV TEXAS,DENT BRANCH,DEPT MICROBIOL,6516 JOHN FREEMAN AVE,POB 20068HOUSTON TX 77225 UNIV TEXAS,DENT BRANCH,DEPT MICROBIOL,6516 JOHN FREEMAN AVE,POB 20068HOUSTON TX 77225 ISIS PHARMACEUT CARLSBAD CA 92008
Titolo Testata:
Cancer research
fascicolo: 10, volume: 53, anno: 1993,
pagine: 2330 - 2337
SICI:
0008-5472(1993)53:10<2330:EOHPTA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
OPEN READING FRAMES; HUMAN KERATINOCYTES; VIRUS-REPLICATION; GENE-EXPRESSION; TYPE-16; DNA; E7; E6; IDENTIFICATION; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
C. Steele et al., "EFFECTS OF HUMAN PAPILLOMAVIRUS TYPE-18-SPECIFIC ANTISENSE OLIGONUCLEOTIDES ON THE TRANSFORMED PHENOTYPE OF HUMAN CARCINOMA CELL-LINES", Cancer research, 53(10), 1993, pp. 2330-2337

Abstract

DNA of human papillomavirus type 18 is present in several human cancer cell lines thal were derived from oral or cervical tumors, and it isknown that several features of the transformed phenotype can be inhibited by expression Of antisense RNA to human papillomavirus (HPV). Thepre ent study was performed to find out whether antisense oligonucleotides were also inhibitory. Synthetic oligonucleotides were made that were complementary to regions of the start codons of the E6 and E7 genes of HPV-18. These were added to cultures of the oral cancer cell line 1483 and the cervical cancer cell line C4-1, each of which contain DNA of HP-18. As controls we used the oral cancer cell line 183 and themonkey kidney cell line Vero, which do not contain HPV. Anti-E6 and anti-E7 oligonucleotides, in concentrations between 1 and 5 muM, significantly inhibited the growth of the 1483 and C4-1 cells, but not the 183 or Vero cell lines. Treatment of the 1483 cells with a combination of 2.5 muM of each of the antisense oligonucleotides was a more effective inhibitor than 5 muM of either one used alone. Antisense oligonucleotides had no effect on the ability of 1483 cells to form foci in soft agar, nor on their plating efficiency or serum requirements. Microscopic examination of 1483 cells showed that the antisense E7 oligonucleotide produced cell-rounding, detachment from the surface of the culture flask, and cell death, while the antisense E6 oligonucleotide had none of these effects. Random-sequence oligonucleotides had no effects of any type on any cells that were growing in culture. However, if random-sequence oligonucleotides were added to cells at the time they were passed to a new culture vessel, they produced severe nonspecific toxic effects. These results show that the use of synthetic oligonucleotides is an effective way of producing antisense-mediated changes in thebehavior of human cancer cells that contain DNA of HPV-18.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 12:43:50