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Titolo:
DIFFERENTIATION OF SPECIES OF THE GENUS ORTHOPOXVIRUS IN A DOT-BLOT ASSAY USING DIGOXIGENIN-LABELED DNA-PROBES
Autore:
MEYER H; OSTERRIEDER N; PFEFFER M;
Indirizzi:
SANITATSAKAD BUNDESWEHR,INST MIKROBIOL,NEUHERBERGSTR 11 W-8000 MUNICH45 GERMANY FED ARMED FORCES MED ACAD,INST MICROBIOL MUNICH GERMANY INST MED MICROBIOL INFECT & EPIDEM DIS MUNICH GERMANY
Titolo Testata:
Veterinary microbiology
fascicolo: 4, volume: 34, anno: 1993,
pagine: 333 - 344
SICI:
0378-1135(1993)34:4<333:DOSOTG>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
COWPOX VIRUS; MONOCLONAL-ANTIBODIES; POXVIRUS INFECTION; GENOME STRUCTURE; PROTEIN; ECTROMELIA; CAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
H. Meyer et al., "DIFFERENTIATION OF SPECIES OF THE GENUS ORTHOPOXVIRUS IN A DOT-BLOT ASSAY USING DIGOXIGENIN-LABELED DNA-PROBES", Veterinary microbiology, 34(4), 1993, pp. 333-344

Abstract

A dot blot assay using five different digoxigenin-labeled probes was established for specific detection and differentiation of four speciesbelonging to the genus Orthopoxvirus. As little as 20 pg orthopoxvirus DNA can be detected, corresponding to approximately 8 x 10(4) DNA molecules. A total of 37 orthopoxvirus strains and isolates of differentorigin were investigated and could be assigned to the species vaccinia, cowpox, camelpox, and mousepox virus. These findings agree with their identification by biological means. Additionally, a simple procedure to isolate orthopoxvirus DNA directly from scab material was developed. This allows a fast classification without isolating the virus.

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Documento generato il 30/11/20 alle ore 10:16:03