Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
LYMPHOCYTE MIGRATION ACROSS HIGH ENDOTHELIUM IS ASSOCIATED WITH INCREASES IN ALPHA-4-BETA-1 INTEGRIN (VLA-4) AFFINITY
Autore:
HOURIHAN H; ALLEN TD; AGER A;
Indirizzi:
UNIV MANCHESTER,DEPT CELL & STRUCT BIOL,IMMUNOL GRP,OXFORD RD MANCHESTER M13 9PT LANCS ENGLAND UNIV MANCHESTER,DEPT CELL & STRUCT BIOL,IMMUNOL GRP,OXFORD RD MANCHESTER M13 9PT LANCS ENGLAND CHRISTIE HOSP & HOLT RADIUM INST,NHS TRUST,PATERSON INST CANC RES MANCHESTER M20 9BX LANCS ENGLAND
Titolo Testata:
Journal of Cell Science
, volume: 104, anno: 1993,
parte:, 4
pagine: 1049 - 1059
SICI:
0021-9533(1993)104:<1049:LMAHEI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HOMING RECEPTORS; T-CELLS; TRANSENDOTHELIAL MIGRATION; ADHESION MOLECULES; VENULE CELLS; NODES; RAT; RECIRCULATION; FIBRONECTIN; MECHANISMS;
Keywords:
LYMPHOCYTE; HIGH ENDOTHELIUM; TRANSENDOTHELIAL MIGRATION; ALPHA-4-BETA-1 INTEGRIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
H. Hourihan et al., "LYMPHOCYTE MIGRATION ACROSS HIGH ENDOTHELIUM IS ASSOCIATED WITH INCREASES IN ALPHA-4-BETA-1 INTEGRIN (VLA-4) AFFINITY", Journal of Cell Science, 104, 1993, pp. 1049-1059

Abstract

The constitutive recirculation of lymphocytes between the widely distributed organs of the immune system is essential for host defence. We have developed an in vitro model of lymphocyte migration from the blood into lymph nodes which employs primary cultures of high endothelial cells (HEC). HEC-adherent lymphocytes adopt one of two distinct morphologies which correlates with their position in the endothelial layer; type I cells are bound to the surface of HEC and type II cells are underneath the endothelial layer. In a previous study we reported that the numbers of type I and type II cells are independently regulated, however the relationship between these two lymphocyte populations was notdetermined. In this study we have carried out detailed kinetic, phenotypic and functional analyses of type I and type II lymphocytes and determined their relationship. Using allotype marked lymphocytes from the PVG.RT7a and PVG.RT7b rat strains in a pulse-chase analysis, type I and type II lymphocytes were found to represent the same population oflymphocytes at different stages of interaction with the endothelial layer, rather than representing two independent lymphocyte populations. Migration was an irreversible event and the efficiency of migration (i.e. transition from type I to type II) was related to the concentration of lymphocytes plated on to the HEC layer. Following transmigrationlymphocytes showed an increased ability to migrate across HEC layers and to bind to immobilised CSI peptide. The increased binding to CSI peptide was transient and fell to control levels over a 3 hour time period. The expression of alpha4 integrin subunit on lymphocytes was unchanged following migration which suggests that the affinity of the CSI receptor, alpha4beta1, is upregulated by interaction with HEC. Together these results suggest that transendothelial migration is regulated by increases in the affinity of alpha4beta1 integrin on lymphocytes following contact with HEC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 15:12:01