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Titolo:
HYDROPHOBIC CHARACTERIZATION OF THERMOPHILIC CAMPYLOBACTER SPECIES AND ADHESION TO INT-407 CELL-MEMBRANES AND FIBRONECTIN
Autore:
MOSER I; SCHRODER W;
Indirizzi:
FREE UNIV BERLIN,INST MIKROBIOL & TIERSEUCHEN,FABECKSTR 36A D-14195 BERLIN GERMANY FREE UNIV BERLIN,INST BIOCHEM D-14195 BERLIN GERMANY
Titolo Testata:
Microbial pathogenesis
fascicolo: 3, volume: 22, anno: 1997,
pagine: 155 - 164
SICI:
0882-4010(1997)22:3<155:HCOTCS>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
JEJUNI SURFACE-PROTEINS; INVITRO BINDING; INTERNALIZATION; ADHERENCE; COLI;
Keywords:
THERMOPHILIC CAMPYLOBACTER; HYDROPHOBICITY; ADHESION; INT 407 CELLS; FIBRONECTIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
20
Recensione:
Indirizzi per estratti:
Citazione:
I. Moser e W. Schroder, "HYDROPHOBIC CHARACTERIZATION OF THERMOPHILIC CAMPYLOBACTER SPECIES AND ADHESION TO INT-407 CELL-MEMBRANES AND FIBRONECTIN", Microbial pathogenesis, 22(3), 1997, pp. 155-164

Abstract

Cell surface hydrophobicity of Campylobacter jejuni, C. coli, C. lariand C. upsaliensis was tested by hydrophobic interaction chromatography on octylsepharose CL-4B. The hydrophobicity was influenced by cultivation mode, presence or absence of intact lipopolysaccharide (LPS) and outer membrane protein structures. Species-specific differences of hydrophobic characteristics were not detected. Bacteria grown in fluid medium exhibited a high degree of hydrophobicity. Agar-grown bacteria showed hydrophobic interaction to a significant lower extent. By oxidation of LPS with sodium meta-periodate the hydrophobicity of agar-grown bacteria was slightly increased. Bacteria pretreated with proteinaseK exhibited a marked decrease of hydrophobic interaction, whereas pretreatment with trypsin did not influence the hydrophobic interaction. Live bacteria were allowed to adhere to INT407 cell membranes. With exception of one aflagellate strain, bacteria grown in fluid medium adhered better to the cellular substrate than agar-grown bacteria. This difference was not found when adhesion to fibronectin was tested. LPS-oxidized bacteria adhered significantly better to both cell membranes and fibronectin, whereas proteinase K treated bacteria exhibited a significant loss of adhesion capacity for both substrates. (C) 1997 Academic Press Limited.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/20 alle ore 07:07:09