Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
RELEASE OF NEUROTRANSMITTERS AND NEUROSECRETORY SUBSTANCES DURING INVITRO MATURATION OF MOUSE HYPOTHALAMIC CULTURES
Autore:
MIRANDACONTRERAS L; MENDOZA RV; ZAMBRANO E; PALACIOSPRU EL;
Indirizzi:
UNIV LOS ANDES,CTR MICROSCOPIA ELECTR,APARTADO POSTAL 163 MERIDA 5101VENEZUELA UNIV LOS ANDES,CTR MICROSCOPIA ELECTR,APARTADO POSTAL 163 MERIDA 5101VENEZUELA INT INST ADV STUDIES CARACAS VENEZUELA
Titolo Testata:
Developmental neuroscience
fascicolo: 5-6, volume: 14, anno: 1992,
pagine: 377 - 385
SICI:
0378-5866(1992)14:5-6<377:RONANS>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; RAT ARCUATE NUCLEUS; CONTAINING NEURONS; ELECTROCHEMICAL DETECTION; HORMONE PRECURSOR; BETA-ENDORPHIN; HUMAN-BRAIN; CELLS; CATECHOLAMINE; SECRETION;
Keywords:
CATECHOLAMINERGIC NEURONS; HYPOTHALAMUS; NEUROSECRETORY ACTIVITY; NEUROTRANSMITTERS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
L. Mirandacontreras et al., "RELEASE OF NEUROTRANSMITTERS AND NEUROSECRETORY SUBSTANCES DURING INVITRO MATURATION OF MOUSE HYPOTHALAMIC CULTURES", Developmental neuroscience, 14(5-6), 1992, pp. 377-385

Abstract

The maturation of the neurosecretory activity of a hypothalamic nervecell population grown in vitro, prepared from 10-day-old mice and cultured for 6 days, has been demonstrated in the present report. A low-molecular weight polypeptide of 30-kD was found to be released into theculture media during the 6-day period of incubation, as analyzed by SDS-polyacrylamide gel electrophoresis. Comparative electrophoresis of the in situ hypothalamic, neurohypophysis and cerebral cortex homogenates revealed the presence of a 30-kD protein component in both the hypothalamus and neurohypophysis but not in the cerebral cortex. The release of the 30-kD polypeptide into the incubation media indicates an expression of the neurosecretory activity of the peptidergic neurons of the hypothalamus during in vitro maturation. On the other hand, high pressure liquid chromatography with electrochemical detection showed appreciable quantities of released dopamine (DA), epinephrine and serotonin (5-HT) in the incubation media in which the neurons were allowed to differentiate. There was a steady release of DA during the 6-day incubation period, varying from 0.21 +/- 0.02 to 0.49 +/- 0.05 ng/mg protein. The epinephrine level increased progressively from day 1 to 6 of culture, from 3.73 +/- 0.57 to 12.08 +/- 1.81 ng/mg protein, respectively. The measured 5-HT level was 0.07 +/- 0.001 on day 2 and increasedto 0.38 +/- 0.05 ng/mg protein on day 6 of culture. These data demonstrate the functional maturation of catecholaminergic, serotoninergic and peptidergic neurons in these rotary histotypic cultures of the mouse hypothalamus.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 02:30:00