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Titolo:
TIME-COURSE DEVELOPMENT OF C-14 SPECIFIC ACTIVITY OF CHLOROPHYLL-A, CARBON, AND PROTEINS IN ALGAL CULTURES
Autore:
RIEMANN B; LIGNELL R; LAWS E;
Indirizzi:
INT AGCY C DETERMINAT,INST WATER QUAL,AGERN ALLE 11 DK-2970 HORSHOLM DENMARK UNIV HELSINKI,DEPT LIMNOL SF-00710 HELSINKI FINLAND UNIV HAWAII MANOA,SCH OCEAN & EARTH SCI & TECHNOL,DEPT OCEANOG HONOLULU HI 96822
Titolo Testata:
Limnology and oceanography
fascicolo: 1, volume: 38, anno: 1993,
pagine: 96 - 111
SICI:
0024-3590(1993)38:1<96:TDOCSA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HIGH PHYTOPLANKTON GROWTH; MARINE-PHYTOPLANKTON; PRODUCTION-RATES; PLANKTON; BIOMASS; WATERS; ATP; METHODOLOGY; NITROGEN; PATTERNS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
B. Riemann et al., "TIME-COURSE DEVELOPMENT OF C-14 SPECIFIC ACTIVITY OF CHLOROPHYLL-A, CARBON, AND PROTEINS IN ALGAL CULTURES", Limnology and oceanography, 38(1), 1993, pp. 96-111

Abstract

The C-14 labeling of Chl a, C, and protein was followed in eight algal batch cultures and three algal continuous cultures. High pressure liquid chromatography and paper chromatography were applied in purifyingChl a. From methodological investigations, we concluded that time-course measurements of C-14 specific activities for Chl a, C, and proteingave similar results as long as turnover of the inorganic C pool reduced the specific activity of inorganic C by less than or similar to 30%. In these cases growth rates estimated from labeling of total algal C overestimated net growth rates by approximately 18%. Chl a C-specific activities consistently exceeded particulate C specific activities in dense cultures when inorganic C turnover reduced the specific activity of the inorganic C by greater than or similar to 65%. The difference in specific activity was positively correlated with the degree of isotope dilution. When the growth rates based on labeling of Chl a were corrected for isotope dilution, they exceeded growth rates estimated from changes in particulate C by approximately 12%, the difference presumably reflecting respiratory loss of algal C. However, even when isotope dilution corrections were applied, growth rates estimated from particulate C labeling consistently underestimated net growth rates when the specific activity of the inorganic C declined by >65%. The degree of underestimation was positively correlated with the extent of isotope dilution.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 23:23:19