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Titolo:
TRYPANOSOME METABOLISM OF MYRISTATE, THE FATTY-ACID REQUIRED FOR THE VARIANT SURFACE GLYCOPROTEIN MEMBRANE ANCHOR
Autore:
DOERING TL; PESSIN MS; HOFF EF; HART GW; RABEN DM; ENGLUND PT;
Indirizzi:
JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM,725 N WOLFE ST BALTIMORE MD21205 JOHNS HOPKINS UNIV,SCH MED,DEPT BIOL CHEM,725 N WOLFE ST BALTIMORE MD21205 JOHNS HOPKINS UNIV,SCH MED,DEPT PHYSIOL BALTIMORE MD 21205
Titolo Testata:
The Journal of biological chemistry
fascicolo: 13, volume: 268, anno: 1993,
pagine: 9215 - 9222
SICI:
0021-9258(1993)268:13<9215:TMOMTF>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHOSPHOLIPASE-C; AFRICAN TRYPANOSOMES; CULTURED FIBROBLASTS; ALPHA-THROMBIN; BRUCEI; BIOSYNTHESIS; PURIFICATION; PROTEIN; IDENTIFICATION; FORM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
T.L. Doering et al., "TRYPANOSOME METABOLISM OF MYRISTATE, THE FATTY-ACID REQUIRED FOR THE VARIANT SURFACE GLYCOPROTEIN MEMBRANE ANCHOR", The Journal of biological chemistry, 268(13), 1993, pp. 9215-9222

Abstract

The trypanosome variant surface glycoprotein (VSG) is anchored to theouter leaflet of the parasite plasma membrane by a glycosyl phosphatidylinositol (GPI). The VSG anchor is unique among GPIs in containing exclusively dimyristoylglycerol as its lipid moiety. Myristate is incorporated into the anchor precursor by sequential deacylation and specific reacylation with myristate. Although myristate is required for the VSG anchor, trypanosomes cannot synthesize this fatty acid and must import their entire supply from the host bloodstream, where it exists inlow abundance. Chemical analysis of these parasites reveals that mostof their myristate is in VSG protein, with no major lipid storage form. Unexpectedly, when these cells are radiolabeled with [H-3]myristatein culture, most of the label is incorporated into phospholipids, with little into VSG. This apparent contradiction is explained by the fact that trypanosomes in culture medium elongate much of the [H-3]myristate into palmitate and stearate, probably because the medium (with only 5% serum) contains limiting amounts of these fatty acids. In contrast, trypanosomes radiolabeled in whole blood (with higher concentrations of palmitate and stearate) do not modify most of the [H-3]myristate,and instead utilize the major portion of it for GPI synthesis. Our studies suggest that bloodstream trypanosomes have evolved highly efficient means of directing myristate into the GPI biosynthetic pathway.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 21:48:49