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Titolo:
DELTA-OPIOID RECEPTOR MOBILIZATION OF INTRACELLULAR CALCIUM IN SH-SY5Y CELLS - LACK OF EVIDENCE FOR DELTA-RECEPTOR SUBTYPES
Autore:
CONNOR MA; KEIR MJ; HENDERSON G;
Indirizzi:
UNIV BRISTOL,DEPT PHARMACOL,UNIV WALK BRISTOL BS8 1TD AVON ENGLAND UNIV BRISTOL,DEPT PHARMACOL BRISTOL BS8 1TD AVON ENGLAND
Titolo Testata:
Neuropharmacology
fascicolo: 1, volume: 36, anno: 1997,
pagine: 125 - 133
SICI:
0028-3908(1997)36:1<125:DRMOIC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHARMACOLOGICAL CHARACTERIZATION; DIFFERENTIAL ANTAGONISM; FUNCTIONAL EXPRESSION; ADENYLYL-CYCLASE; MU; DESENSITIZATION; CLONING; NALTRINDOLE; AFFINITY; AGONISTS;
Keywords:
SH-SY5Y NEUROBLASTOMA CELLS; DELTA-OPIOID RECEPTOR; DELTA-RECEPTOR SUBTYPES; CA2+ MOBILIZATION; HOMOLOGOUS DESENSITIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
M.A. Connor et al., "DELTA-OPIOID RECEPTOR MOBILIZATION OF INTRACELLULAR CALCIUM IN SH-SY5Y CELLS - LACK OF EVIDENCE FOR DELTA-RECEPTOR SUBTYPES", Neuropharmacology, 36(1), 1997, pp. 125-133

Abstract

delta-opioid receptor agonists mobilize intracellular Ca2+([Ca2+](i))in SH-SY5Y cells when applied in the presence of muscarinic agonists. The putative delta 1 receptor agonist [D-Pen(2),D-Pen(5)]-enkephalin (DPDPE) elevated [Ca2+](i) with an EC(50) of 11 nM and the putative delta 2 agonist deltorphin II elevated [ca(2+)](i), with an EC(50) of 14nM. The maximal elevations of [Ca2+](i) caused by both agonists were not different, nor were maximally effective concentrations of DPDPE (1mu M) and deltorphin II (1 mu M) applied together more effective thaneither agonist applied alone. The elevations of [Ca2+](i) caused by DPDPE (1 mu M) and deltorphin II (1 mu M), in the presence of carbachol, desensitized rapidly with continued opioid exposure and the cross-desensitization between DPDPE and deltorphin II was complete. The putative delta 1 selective antagonist 7-benzylidenenaltrexone (BNTX) and theputative delta 2 selective antagonist naltriben both reduced the elevations of [Ca2+](i) caused by DPDPE (30 nM) and deltorphin II (10 nM),by greater than 50% at concentrations of less than 10 nM. In SH-SY5Y cells delta-receptor mediated elevation of [Ca2+](i) is mediated by a population of receptors, which does not discriminate between agonists and antagonists purportedly selective for delta 1 or delta 2 receptors. (C) 1997 Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:44:16