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Titolo:
INCREASED PO GLYCOPROTEIN GENE-EXPRESSION IN PRIMARY AND TRANSFECTED RAT SCHWANN-CELLS AFTER TREATMENT WITH AXOLEMMA-ENRICHED FRACTION
Autore:
KNIGHT RM; FOSSOM LH; NEUBERGER TJ; ATTEMA BL; TENNEKOON G; BHARUCHA V; DEVRIES GH;
Indirizzi:
VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT BIOCHEM & MOLEC BIOPHYS,1101 E MARSHALL RICHMOND VA 23298 VIRGINIA COMMONWEALTH UNIV,MED COLL VIRGINIA,DEPT BIOCHEM & MOLEC BIOPHYS,1101 E MARSHALL RICHMOND VA 23298 UNIV MICHIGAN,DEPT PEDIAT NEUROL ANN ARBOR MI 48109
Titolo Testata:
Journal of neuroscience research
fascicolo: 1, volume: 35, anno: 1993,
pagine: 38 - 45
SICI:
0360-4012(1993)35:1<38:IPGGIP>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA LEVELS; T-ANTIGEN GENE; PERIPHERAL MYELIN; CYCLIC-AMP; PROTEIN; NERVE; AXONS; EXTRACTION; INHIBITION; CULTURES;
Keywords:
DIFFERENTIATION; GLIAL CELLS; MYELIN PROTEINS; NEURON-GLIAL INTERACTION; MYELIN PROTEIN GENE EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
R.M. Knight et al., "INCREASED PO GLYCOPROTEIN GENE-EXPRESSION IN PRIMARY AND TRANSFECTED RAT SCHWANN-CELLS AFTER TREATMENT WITH AXOLEMMA-ENRICHED FRACTION", Journal of neuroscience research, 35(1), 1993, pp. 38-45

Abstract

To elucidate the role of axonal plasma membrane factors in the differentiation of Schwann cells, we investigated the effect of an axolemma-enriched fraction (AEF) isolated from myelinated CNS tissue on the expression of PO glycoprotein, the major glycoprotein in peripheral myelin, in primary rat Schwann cells (PSC) isolated from sciatic nerve, as well as in a transfected rat Schwann cell line (TSC). AEF increased PO-mRNA levels in PSC and TSC in a concentration-dependent manner, producing a maximal induction of nearly twofold after 48 hr of treatment. Asimilar induction of PO mRNA was elicited in TSC by the cAMP-activating agents 8-bromo-cAMP and forskolin, which have been shown to induce myelin proteins in PSC. In addition to inducing PO mRNA, AEF and forskolin also increased the amount of PO protein in TSC, as indicated by increased PO-immunoreactive staining. However, in TSC, axolemma caused no increase in expression of CAT linked to a PO promoter while forskolin caused a marked increase in the expression from the PO promoter. These results suggest that AEF, in contrast to forskolin, does not regulate PO-mRNA expression at the level of transcriptional activity. Thesein vitro systems may be useful for the study of axolemmal factors that induce Schwann cell differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/07/20 alle ore 05:26:45