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Titolo:
INITIAL LOCALIZATION OF REGULATORY REGIONS OF THE CARDIAC SARCOLEMMALNA-CA2+ EXCHANGER()
Autore:
MATSUOKA S; NICOLL DA; REILLY RF; HILGEMANN DW; PHILIPSON KD;
Indirizzi:
UNIV TEXAS,SW MED CTR,DEPT PHYSIOL DALLAS TX 75235 UNIV CALIF LOS ANGELES,SCH MED,DEPT MED LOS ANGELES CA 90024 UNIV CALIF LOS ANGELES,SCH MED,DEPT PHYSIOL LOS ANGELES CA 90024 UNIV CALIF LOS ANGELES,SCH MED,CARDIOVASC RES LAB LOS ANGELES CA 90024 YALE UNIV,SCH MED,DEPT INTERNAL MED NEW HAVEN CT 06510
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 9, volume: 90, anno: 1993,
pagine: 3870 - 3874
SICI:
0027-8424(1993)90:9<3870:ILORRO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
XENOPUS OOCYTES; EXPRESSION; CLONING; CALCIUM;
Keywords:
EXCHANGER INHIBITORY PEPTIDE; GIANT EXCISED PATCH; OOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
S. Matsuoka et al., "INITIAL LOCALIZATION OF REGULATORY REGIONS OF THE CARDIAC SARCOLEMMALNA-CA2+ EXCHANGER()", Proceedings of the National Academy of Sciences of the United Statesof America, 90(9), 1993, pp. 3870-3874

Abstract

We have analyzed the regulatory properties of the wild-type cardiac Na+-Ca2+ exchanger expressed in Xenopus laevis oocytes using the giant excised patch technique. The exchanger is activated by cytoplasmic application of chymotrypsin and exhibits a number of properties that can be changed or abolished by chymotrypsin treatment, including cytoplasmic Na+-dependent inactivation, secondary regulation by free cytoplasmic Ca2+, and inhibition by exchanger inhibitory peptide. Thus, the cloned exchanger expressed in oocytes exhibits regulatory properties similar to those of the native sarcolemmal exchanger. The exchanger proteincontains a large (520 amino acids) hydrophilic domain modeled to be intracellular. The role of this region in exchanger function and regulation was examined by deletion mutagenesis. Mutants with residues 240-679 and 562-685 deleted exhibited exchange activity, indicating that this extensive region is not essential for transport function. Both mutants were stimulated by chymotrypsin treatment. Neither mutant demonstrated regulation by free cytoplasmic Ca2+ (Ca(i)2+) or inhibition by exchanger inhibitory peptide (XIP). However, mutant DELTA562-685 but notDELTA240-679 displayed Na+-dependent inactivation. The data suggest that the binding sites for XIP and regulatory Ca2+ may reside in the region encompassed by residues 562-685. A chimera made from renal and cardiac exchangers has normal regulatory characteristics and helps to further define these sites.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/10/20 alle ore 10:51:59